EMBRIOGENESIS SOMATIK ANGGREK BULAN Phalaenopsis amabilis (L.) Bl: STRUKTUR DAN POLA PERKEMBANGAN

Edy Setiti Wida Utami (Laboratorium Biologi Reproduksi, Fakultas MIPA UNAIR)
Issirep Soemardi (Laboratorium Anatomi Tumbuhan, Fakultas Biologi UGM)
Taryono Taryono (Laboratorium Kultur Jaringan Tumbuhan, Fakultas Pertanian UGM)
Endang Semiarti (Laboratorium Kultur Jaringan Tumbuhan, Fakultas Biologi UGM)

Berkala Penelitian Hayati
Vol 13 No 1 (2007): December 2007

Research of the structure and development pattern of somatic embryos from callus of leaf explants moon orchid Phalaenopsis amabilis (L) Bl had been done. One year old of plantlets were used as explants sources. Basal leaf of these explants were cultured in Somatic Embryo Induction Medium (SEIM) e.i.: NP(New Phalaenopsis) medium added with 2 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Then somatic embryos were transferred to EMM (Embryo Maturation Medium) e.i.: NP medium added with 1 mg/L NAA, 1 mg/L BA, 10 g/L sucrose, and 2 g/L gellan gum. Finally, mature somatic embryo were transferred to NP medium without plant growth regulator as Embryo Germination Medium (EGM). The origin of somatic embryos initially from single cell at the pheriphery of embryogenic callus. These cells then devided in mitotic repeatedly formed globular proembryo, elongation embryo, and completed embryo. The structure and development pattern of somatic embryos as the same as with zygotic embryo.