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All Journal Natural Science: Journal of Science and Technology Agrotrop : Journal on Agriculture Science BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Pelita Perkebunan Journal of Tropical Biodiversity and Biotechnology Biosaintifika: Journal of Biology & Biology Education Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Penelitian Hayati Jurnal Kreativitas dan Inovasi (Jurnal Kreanova)
Edy Setiti Wida Utami
Biology Department, Faculty Of Sciences And Technology, Airlangga University, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Social Sciences Veterinary Other

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The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro Utami, Edy Setiti Wida; Hariyanto, Sucipto
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.5165

Abstract

Dendrobium antennatum has high economic value as cut flowers and flowerpots. Like orchid seeds in general, D. antennatum is difficult to germinate under natural conditions. This study aimed to determine the effect of coconut water on seed germination and embryo development, as well as the effect of NAA on shoots development of D. antennatum. This study consisted of two stages. In the first stage, the 12 weeks-old seeds after pollination were sown on MS medium containing 2 g/L peptone + 0%; 5%; 10%; and 20% coconut water. After 8 weeks of culture, the seeds germinated and the shoot formed were recorded. The highest in seed germination (92.2%) and the formation of shoots (51.4%) were obtained when seeds were cultured on MS medium containing 2 g/L peptone + 20% coconut water. In the second stage, the shoots were sub-cultured on MS medium containing 1 mg/L thidiazuron + 0 mg/L; 1 mg/L; 2 mg/L; and 3 mg/L NAA. After 16 weeks of sub-culture, the height of plantlets, the length of the roots and leaves, number of leaves and roots formed were recorded. MS medium containing 1 mg/L thidiazuron + 1 mg/L NAA was the most suitable for the shoots development of D.antennatum. The embryo development of D.antennatum in vitro begins with the enlargement of embryo, with further it emerges from the seed coat (germinated) followed by the formation of the apical meristems to form the shoots and the roots.How to CiteUtami, E. S. W., & Hariyanto, S. (2016). The Effect of Organic Nutrient and Growth Regulators on Seed Germination, Embryo and Shoots Development of Dendrobium antennatum Lindl. Orchid by In Vitro. Biosaintifika: Journal of Biology & Biology Education, 8(2), 165-171.
Callus Induction of Gendarussa (Justicia gendarussa) by Various Concentration of 2,4-D, IBA, and BAP Wahyuni, Dwi Kusuma; Andriani, Putri; Ansori, Arif Nur Muhammad; Utami, Edy Setiti Wida
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 3 (2017): December 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i3.11347

Abstract

Justicia gendarussa Burm.f., a medicinal plant, is Acanthaceae that has many functions. Furthermore, the compounds in gendarussa must be produced in high quantity and quality by applying callus culture method. Accordingly, it is important to study the effects of plant growth regulators (2,4-D, IBA, and BAP) on callus induction of gendarussa leaves. This research design utilized a factorial design with two factors (2,4-D and IBA: 0.5, 1, 1.5 mg/L and BAP: 0.5, 1, 1.5, 2 mg/L). The experiment consisted of 24 treatments, each of which was repeated 3 times. Observation was carried out in 6 weeks. Data on the time of callus formation, percentage of explants formed callus, and callus morphology were analyzed descriptively,while data on fresh and dry weight were analyzed by Two-Way ANOVA (? = 0.5). Interestingly, the results showed that various concentration of plant growth regulators (2,4-D, IBA, and BAP) affected callus induction from leaf explants of gendarussa. We concluded that the most optimal treatment combination of concentration of plant growth regulators in inducing callus from leaf explants of gendarussa is 1.5 mg/L 2,4-D and 2 mg/L BAP with a relatively long period of callus formation at the earliest, i.e. on day 5, 2.247 g of fresh weight, 0.108 gof dry weight, white callus translucent, and friable. Moreover, the optimum treatment will be used to produce secondary metabolite and seed synthetic by cell suspension culture.
Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510 Wahyuni, Dwi Kusuma; Hafida, Siti Nur; Ermayanti, Tri Muji; Wardoyo, Bambang Prajoga Eko; Purnobasuki, Hery; Utami, Edy Setiti Wida
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.8223

Abstract

Gandarusa (Justicia gendarussa Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of Agrobacterium rhizogenes strain LB510 on hairy roots induction of gandarusa (Justicia gendarussa Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD600 = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of Agrobacterium rhizogenes strain LB510 was affected the lenght of hairy roots induction of J. gendarussa Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD600 0.2 was the best treatment for hairy root induction on Justicia gendarussa Burm. f. This data could be used for optimized the quality of methode of hairy root induction.
Callus Induction of Piper betle L. Var Nigra Using 2,4-Dichlorofenoxyacetic Acidand 6-Benzil Aminopurin Junairiah, Junairiah; Purnomo, Purnomo; Utami, Edy Setiti Wida; Ni’matuzahroh, Ni’matuzahroh; Sulistyorini, Lilis
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.143 KB) | DOI: 10.15294/biosaintifika.v10i3.15962

Abstract

Piper betle L var Nigra (black betel) is a member of Piperaceae family which has potential as medicinal plant due to its secondary metabolites. Callus culture is one of the alternative methods to elevate production of secondary metabolites. This study was aimed to determine the effect of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzil aminopurine (BAP) towards callus induction and growth, also to determine the most optimal variation of 2,4-D and BAP concentration for callus induction of black betel leaf explant. This study was a laboratory experimental study with complete randomized design. Black betel leaf explant was planted in Murashige and Skoog (MS) medium supplemented with 2,4-D and BAP growth regulators at concentration of 0.0 mg/L, 0.5 mg/L, 1.0 mg/L, 1.5 mg/L, 2.0 mg/L respectively. Parameter recorded for callus induction and growth including callus induction time (days), percentage of explant forming callus, fresh weight, dry weight, color and texture. After callus planted for 8 weeks, analysis was performed statistically. Result showed that 2,4-D and BAP supplementation to medium affected the growth of black bete l leaf explants. Additional concentration of 0.5 mg/L 2,4-D and 1.0 mg/L BAP growth regulators showed the fastest response in callus formation, at 7.25 days. Growth regulators of 2,4-D 0.5 mg/L and BAP 2.0 mg/L concentration produced the highest fresh and dry weight, at 0.6802 g and 0.0670 g respectively. The best treatment was used as a basis to produce secondary metabolites.
INDUKSI REGENERASI PHALAENOPSIS AMABILIS (L.) BL PADA BERBAGAI DOSIS ZAT PENGATUR TUMBUH Utami, Edy Setiti Wida; Hariyanto, Sucipto
Natural Science: Journal of Science and Technology Vol 4, No 2 (2015): Volume 4 Number 2 (August 2015)
Publisher : Univ. Tadulako

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Abstract

Penelitian bertujuan untuk mengetahui pengaruh 2iP, NAA dan kombinasinya untuk regenerasi anggrek P. amabilis. Potongan ruas dan buku-buku tangkai bunga dikultur dalam media New Phalaenopsis (NP) diberi zat pengatur tumbuh 2iP dan NAA dengan konsentrasi 0.0:0.0; 0.0:2.5; 0.5:2.0; 1.0:1.5; 1.5:1.0; 2.0:0.5 dan 2.5:0.0 mg L-1. Setiap 4 minggu, ruas dan buku-buku disubkultur pada media yang sama selama 12 minggu. Pengamatan terhadap waktu inisiasi kalus dilakukan setiap hari, waktu inisiasi tunas dan akar dilakukan setiap seminggu sekali. Penghitungan jumlah tunas dan akar dilakukan pada minggu ke 12 setelah inokulasi. Berdasarkan hasil penelitian dapat disimpulkan bahwa regenerasi tunas dari ruas tangkai bunga P. amabilis terbentuk secara tidak langsung melalui fase kalus. Regenerasi tunas dan akar dari buku-buku terbentuk tanpa melalui fase kalus. Perlakuan kombinasi zat pengatur tumbuh (2iP dan NAA) memberikan pengaruh yang lebih baik terhadap regenerasi daripada perlakuan zat pengatur tumbuh tunggal atau tanpa zat pengatur tumbuh. Buku-buku menghasilkan regenerasi tunas yang lebih tinggi daripada ruas.
Callus induction and secondary metabolite profile from Elephantopus scaber L. Junairiah Junairiah; Diah Ayu Wulandari; Edy Setiti Wida Utami; Nabilah Istighfari Zuraidassanaaz
Journal of Tropical Biodiversity and Biotechnology Vol 6, No 1 (2021): April
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.59234

Abstract

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol.  Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.
Optimasi Isolasi Protoplas Mesofil Daun Anggrek Paraphalaenopsis laycockii EDY SETITI WIDA UTAMI; SUCIPTO HARIYANTO
Agrotrop : Journal on Agriculture Science Vol 5 No 1 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (561.546 KB)

Abstract

Optimalization protoplast isolation of the leaves mesophyll orchid Paraphalaenopsis layockii. The obyectives of the research was to investigate the effect of combination treatment of sucrose concentrations and incubation time on protoplast isolation of the leaves mesophyll orchid of Paraphalaenopsis layockii. The factorial experiment consisted of two factors that was arranged by completely randomized design (CRD) with ten replications. The first factor was concentrations of sucrose which consists of three levels namely 0.4 M, 0.5 M and 0.6 M. The second factor was incubation time which consists of four levels namely 2 hours, 4 hours, 6 hours and 8 hours. Data were analyzed using Kruskal-Wallis test followed by Mann-Whitney test. The results showed that there was effect of treatment for all variables observed. The highest protoplast density and number of viable protoplasts achieved in the combined treatment of 0.6 M sucrose and 4 hours of incubation time.
Pengaruh Pemberian Ekstrak Pisang pada Media VW terhadap Induksi Akar dan Pertumbuhan Tunas Dendrobium lasianthera J.J.Sm EDY SETITI WIDA UTAMI; SUCIPTO HARIYANTO; Y. SRI WULAN MANUHARA
Agrotrop : Journal on Agriculture Science Vol 6 No 1 (2016)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The Effect of Banana Extract at The VW Media on Root Induction and Growth of ShootDendrobium Lasianthera J.J.Sm. Orchid has economic potential as a non-oil exportcommodities which can increase foreign exchange. In addition to supporting industry as cutflowers and potted plants, Dendrobium orchid in particular has been widely recognized as atraditional medicine. Orchids can be propagated in vitro using seed. The main problem in thepropagation of orchid through seed culture is difficulty in root formation. In this study, weevaluated the effect of organic compounds of the banana extract at Vacin and Went mediumcontaining peptone 2 gL-1 to induce growth of root and shoot of the Dendrobium lasianthera.The shoot measuring about 0.3-1.0 cm contain of 1-2 leaves obtained from the seedgermination was cultured on VW medium containing 2 gL-1 peptone additives with 50 gL-1,100 gL-1 and 150 gL-1 banana extract, and without banana extract was used as control. After16 weeks of culture, number of roots, number of leaves, root length, leaf length, the shootheight, root and leaf widest diameters were recorded. Data were statistically analyzed usingANOVA and continued by honestly significance difference (HSD). The results showed thatthe extract of banana statistically gave a significant effect on root induction and growth ofshoot D. lasianthera.
SEED GERMINATION OF Avicennia marina (Forsk.) Vierh. BY PERICARP REMOVAL TREATMENT Hery Purnobasuki; Edy Setiti Wida Utami
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 23 No. 2 (2016)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2016.23.2.349

Abstract

Avicennia marina is one species used for building coastal ecosystem stabilization in Surabaya shores because of its tolerance to high salinity level and resistance to wave energy. Their existence is threatened by various factors i.e. land conversion, pollution and interisland bridge existence.  Growth of A. marina is currently limited by seed dormancy. In an effort to increase propagation efficiency, seed viability and vigor were compared based on collection dates and pericarp removal methods. The results showed no relationship between salinity levels of soaking water or seed buoyancy and seed viability or seed vigor. Collection dates may influence seed viability and vigor. Seeds collected in October and November germinated more quickly than those collected in December, although there were no differences in total germination. Hand-peeled seeds germinated more slowly than soaked seeds for all collections, but with no differences in total germination. Seeds collected in October and November grew more vigorously than those collected in December.
Pengaruh Ekstrak Yeast dan Pisang Raja Terhadap Pertumbuhan Tunas Embrio Vanda hookeriana Rchb.f. Shilfiana Rahayu; Edy Setiti Wida Utami; Aldy Bahaduri Indraloka
Al-Kauniyah: Jurnal Biologi Vol 14, No 1 (2021): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v14i1.16713

Abstract

AbstrakVanda hookeriana Rchb.f. merupakan anggrek yang dilindungi dan keberlangsungan hidupnya di alam terancam punah, sehingga perbanyakan anggrek secara in vitro sangat diperlukan. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak yeast dan pisang raja terhadap pertumbuhan tunas embrio anggrek V. hookeriana Rchb.f. Desain penelitian ini menggunakan RAL faktorial, faktor pertama adalah ekstrak yeast dengan 3 taraf: 0,5 g/L, 1 g/L, dan 1,5 g/L, faktor kedua adalah buah pisang raja dengan 3 taraf: 50 g/L, 100 g/L, dan 150 g/L. Penelitian dilakukan menggunakan teknik kultur jaringan, pengamatan dilakukan setiap bulan sekali dengan mengamati aspek morfologi plantlet. Media yang digunakan adalah Vacin-Went (VW) + kombinasi ekstrak yeast dan pisang raja. Parameter pengamatan berupa tinggi tunas, berat kering tunas, jumlah daun, diameter daun, panjang daun, berat kering daun, jumlah akar, diameter akar, panjang akar, berat kering akar, dan morfologi tunas. Hasil analisis menunjukkan bahwa perlakuan pisang raja 150 g/L + ekstrak yeast 0,5 g/L merupakan konsentrasi yang paling berperan terhadap parameter pertumbuhan anggrek V. hookeriana Rchb.f. Penelitian ini berhasil membuktikan bahwa penambahan komponen organik dalam media kultur jaringan memberikan respon pertumbuhan dan perkembangan yang baik pada eksplan. Komponen organik membantu proses fisiologis eksplan, karena komponen organik mengandung senyawa potensial yang dibutuhkan selama proses pertumbuhan dan perkembangan. Abstract Vanda hookeriana Rchb.f. is one of protected orchid and its survival in the wild was threatened by extinction, so orchid propagation was needed. This research aimed to determine the effect of yeast and pisang raja extracts to embryonal shoot growth of Vanda hookeriana Rchb.f. Experimental design used factorial RAL, first factor was yeast extract with 3 treatments (0.5 g/L; 1 g/L; & 1.5 g/L). Second factor was pisang raja with 3 treatments (50 g/L; 100 g/L; & 150 g/L). The research was conducted using in vitro culture techniques, observations were made once a month by observing the plantlet morphological parameters. The medium used was Vacin-Went (VW) + combination of yeast and pisang raja extracts. Observation parameters were shoot height, shoot dry weight, leaf number, leaf diameter, leaf length, leaf dry weight, number of roots, root diameter, root length, root dry weight and shoot morphology. The analysis showed that pisang raja treatment 150 g/L + yeast extract 0.5 g/L was the best concentration for the growth parameters of Vanda hookeriana Rchbf. This research were succeeded in proving that the addition of organic components in tissue culture media gave better response to growth and development of the explants. Organic components helps the explants in physiological ways because of  the organic components contain potential compounds needed during the growth and development process.
Co-Authors Aldy Bahaduri Indraloka Arif Nur Muhammad Ansori Arif Nur Muhammad Ansori Arif Yachya Bambang Prajoga Eko Wardoyo Bambang Prajoga Eko Wardoyo, Bambang Prajoga Eko Diah Ayu Wulandari Dwi Kusuma Wahyuni Dwi Setyo Rini Eka Sugiyarta Endang Semiarti Fitria Ardiyani Hamidah . Hery Purnobasuki Issirep Soemardi Junairiah Junairiah Lilis Sulistyorini Nabilah Istighfari Zuraidassanaaz Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh, Ni’matuzahroh Purnomo Purnomo Purnomo Purnomo Putri Andriani Putri Andriani Shilfiana Rahayu Siti Nur Hafida Siti Nur Hafida, Siti Nur Sucipto Hariyanto Taryono Taryono Tri Muji Ermayanti Tri Muji Ermayanti Y. SRI WULAN MANUHARA Y. Sri. Wulan Manuhara