BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Vol. 24 No. 1 (2017): BIOTROPIA Vol. 24 No. 1 April 2017

Isolation and Characterization of a Molybdenum-Reducing and Phenolic- and Catechol-Degrading Enterobacter sp. Strain Saw-2

Sabullah, Mohd Khalizan (Unknown)
Rahman, Mohd Fadhil (Unknown)
Ahmad, Siti Aqlima (Unknown)
Sulaiman, Mohd Rosni (Unknown)
Shukor, Mohd Shukri (Unknown)
Gansau, Azlan Jualang (Unknown)
Shamaan, Nor Aripin (Unknown)
Shukor, Mohd Yunus (Unknown)



Article Info

Publish Date
22 May 2017

Abstract

Molybdenum is an emerging pollutant worldwide. This study aimed to isolate a molybdenum-reducing bacterium capable of growing on phenolic compounds, specifically phenol and catechol. The screening process was conducted using a microplate assay. The bacterium reduced molybdenum in the form of sodium molybdate to molybdenum blue (Mo-blue). Optimal molybdate reduction occurred within a narrow pH range of 6.3–6.8 and at temperatures between 34–37 °C. Glucose was the most effective carbon source supporting molybdate reduction, while phenol and catechol did not support the reduction process. Additional requirements for molybdate reduction included sodium molybdate concentrations of 15–30 mM and phosphate at 5.0 mM. The Mo-blue produced exhibited an absorption spectrum characterized by a shoulder at 700 nm and a maximum peak near the infrared region at 865 nm. The Mo-reducing bacterium was partially identified as Enterobacter sp. strain Saw-2. The ability of this bacterium to grow on toxic phenolic compounds and detoxify molybdenum suggests strong potential for use in bioremediation applications.

Copyrights © 2017






Journal Info

Abbrev

biotropia

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

Description

BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and ...