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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Siti Aqlima Ahmad, Siti Aqlima
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Biological Remediation of Cyanide: A Review Ibrahim, Karamba Kabiru; Syed, Mohd Arif; Shukor, Mohd Yunus; Ahmad, Siti Aqlima
BIOTROPIA Vol. 22 No. 2 (2015): BIOTROPIA Vol. 22 No. 2 December 2015
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1249.896 KB) | DOI: 10.11598/btb.2015.22.2.393

Abstract

Cyanide and its complexes are produced worldwide as industrial waste or effluents. Biodegradation is considered the most economical and effective method for removing cyanide from the environment. Numerous studies have identified various microorganisms capable of degrading cyanide under natural conditions. Hydrolytic, oxidative, reductive, and substitutive/transfer reactions are among the most common pathways utilized by microorganisms during cyanide degradation. Biodegradation may occur under aerobic or anaerobic conditions, depending on environmental factors. The use of immobilized microorganisms or purified enzymes has also been shown to increase degradation efficiency. Several microbial species, including Klebsiella oxytoca, Corynebacterium nitrophilum, Brevibacterium nitrophilum, Bacillus spp., Pseudomonas spp., Rhodococcus spp., and strain UKMP-5M, have been reported to be highly effective in cyanide biodegradation.
Isolation and Characterization of a Molybdenum-Reducing and Phenolic- and Catechol-Degrading Enterobacter sp. Strain Saw-2 Sabullah, Mohd Khalizan; Rahman, Mohd Fadhil; Ahmad, Siti Aqlima; Sulaiman, Mohd Rosni; Shukor, Mohd Shukri; Gansau, Azlan Jualang; Shamaan, Nor Aripin; Shukor, Mohd Yunus
BIOTROPIA Vol. 24 No. 1 (2017): BIOTROPIA Vol. 24 No. 1 April 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.1.550

Abstract

Molybdenum is an emerging pollutant worldwide. This study aimed to isolate a molybdenum-reducing bacterium capable of growing on phenolic compounds, specifically phenol and catechol. The screening process was conducted using a microplate assay. The bacterium reduced molybdenum in the form of sodium molybdate to molybdenum blue (Mo-blue). Optimal molybdate reduction occurred within a narrow pH range of 6.3–6.8 and at temperatures between 34–37 °C. Glucose was the most effective carbon source supporting molybdate reduction, while phenol and catechol did not support the reduction process. Additional requirements for molybdate reduction included sodium molybdate concentrations of 15–30 mM and phosphate at 5.0 mM. The Mo-blue produced exhibited an absorption spectrum characterized by a shoulder at 700 nm and a maximum peak near the infrared region at 865 nm. The Mo-reducing bacterium was partially identified as Enterobacter sp. strain Saw-2. The ability of this bacterium to grow on toxic phenolic compounds and detoxify molybdenum suggests strong potential for use in bioremediation applications.
Co-Authors Gansau, Azlan Jualang Karamba Kabiru Ibrahim, Karamba Kabiru Mohd Arif Syed, Mohd Arif Mohd Yunus Shukor, Mohd Yunus Rahman, Mohd Fadhil Sabullah, Mohd Khalizan Shamaan, Nor Aripin Shukor, Mohd Shukri Sulaiman, Mohd Rosni