Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology)
Vol 15 No 3 (2015): October 2015

Cytochrome C oxydase 1 gene sequences long-barbel sheatfish, Kryptopterus limpok (Bleeker, 1852) from Kampar River and Indragiri River of Riau Province

Roza Elvyra (Program Studi Biologi, Fakultas MIPA, Universitas Riau)
Dedy Duryadi Solihin (Departemen Biologi, Fakultas MIPA, Institut Pertanian Bogor)



Article Info

Publish Date
12 Oct 2015

Abstract

This study aimed to analyze the sequence of mitochondrial DNA cytochrome c oxydase 1 (Cox-1) gene of the long barbel sheatfish, Kryptopterus limpok. The Cox-1 gene of Kryptopterus limpok from Kampar and Indragiri Rivers, Riau Province was amplified using PCR technique. The length of the Cox-1 gene sequence was 655 bp containing nine nucleotide sites substituted. Four base substitutions were transition and five base was transversion. Nucleotide frequencies of guanine was a few (G:17.2%) and approximately equal in frequencies of cytosine, thymine, adenine (C: 29.5%, T: 27.5%, A: 25.8%), and G+C content (46.7%). The genetic distance of mtDNA Cox-1 gene sequences between Kryptopterus limpok from Kampar and Indragiri river was 0.01. Phylogenetic tree showed that Kryptopterus limpok from Kampar and Indragiri Rivers are in one cluster with 100% bootstrap value. Abstrak Penelitian ini bertujuan untuk menganalisis runutan gen cytochrome c oxydase 1 (Cox-1) DNA mitokondria ikan lais janggut, Kryptopterus limpok. Gen Cox-1 Kryptopterus limpok dari Sungai Kampar dan Indragiri, Provinsi Riau telah diamplifikasi menggunakan teknik PCR. Runutan gen Cox-1 yang diperoleh sepanjang 655 bp, dengan sembilan situs substitusi nukleotida, meliputi empat substitusi transisi dan lima substitusi transversi basa. Frekuensi nukleotida yang sedikit ditemukan pada guanin (G:17,2%), sedangkan frekuensi cytosin, thymin, adenin kurang lebih sepadan (C: 29,5%, T: 27,5%, A: 25,8%), dan kandungan G+C (46,7%). Jarak genetik runutan gen Cox-1 antara Kryptopterus limpok dari Sungai Kampar dan Sungai Indragiri adalah 0,01. Pohon filogeni memperlihatkan bahwa Kryptopterus limpok dari Sungai Kampar dengan Sungai Indragiri membentuk satu kelompok dengan nilai bootstrap 100%.

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Journal Info

Abbrev

jii

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Description

Aims and Scope Aims: Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) aims to publish original research results on fishes (pisces) in fresh, brackish and sea waters including biology, physiology, and ecology, and their application in the fields of fishing, aquaculture, fisheries ...