Gambir Catechins (Uncaria gambir Roxb) Prevent Oxidative Stress in Wistar Male Rats Fed a High-Fat Diet

Mellina Rahmi (Master Program in Biomedical Science, Faculty of Medicine, Universitas Andalas, Padang, Indonesia)
Rauza Sukma Rita (Department of Biochemistry, Faculty of Medicine, Universitas Andalas, Padang, Indonesia)
Husna Yetti (Department of Public Health and Community Medicine, Faculty of Medicine, Universitas Andalas, Padang, Indonesia)

Majalah Kedokteran Andalas
Vol 44, No 7 (2021): Online Desember 2021

Objective: This study aimed to examine how gambir catechins (Uncaria gambir Roxb) affect serum malondialdehyde (MDA) levels and catalase activity in Wistar white male rats fed a high-fat diet. Methods: This is a true experimental study with a post-test-only control group design. The experiment used 30 rats divided into five groups: a negative control group, a positive control group induced by a high-fat diet, and three treatment groups caused by a high-fat diet and given gambir catechins at doses of 10 mg/kg BW/day, 20 mg/kg BW/day, and 40 mg/kg BW/day for 14 days. The TBARs method measures MDA levels, while the colorimetry method measures catalase activity. One-way ANOVA and Post Hoc Tukey HSD are used to analyze the data. Results: The gambir catechin reduced serum MDA and increased catalase activity in male Wistar strain white rats fed a considerably high-fat diet (p<0.05). Conclusion: In mice fed a high-fat diet, catechin gambir was capable of dealing with oxidative damage.