KERAGAMAN GENETIK TIMOHO (Kleinhovia hospita Linn.) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD)

ILG Nurtjahjaningsih (Center for Forest Biotechnology and Tree Improvement)
Lukman Hakim (Balai Besar Litbang Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta)
Yemima Viktory Utomo (Universitas Kristen Duta Wacana)
Anto Rimbawanto (Balai Besar Penelitian dan Pengembangan Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta)
AYPBC Widyatmoko (Balai Besar Litbang Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta)

JURNAL PEMULIAAN TANAMAN HUTAN
Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan

Timoho (Kleinhovia hospita Linn.) has high economic value for medicine and construction. However, information on genetic diversity and conservation genetic have been limited by the lack of genetic data. Acacia mangium (mangium)This study aimed to assess genetic diversity of timoho using RAPD markers. Leaf samples of seedlings were collected from a nursery at CFBTI in Yogyakarta; the seedlings originated from plantation in Gunung Kidul (GK) and arboretum in Faculty of Forestry, GMU (FK). Seven out of 22 screened RAPD markers were stable in amplification and consisted of 61 polymorphic loci; then these markers were used to analyse genetic diversity and population structure of 48 seedlings from GK and FK. The genetic diversity and structure parameters were calculated by GenAlex software. A moderate level of genetic diversity (mean uHE=0,326) and great genetic distance between the two populations (DA=0.119) were found in this species. PCoA analysis showed that the 48 individuals were divided into two clusters according to their originated population. Appropriate conservation strategies were proposed to protect genetic diversity of this species.