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KERAGAMAN GENETIK TIMOHO (Kleinhovia hospita Linn.) BERDASARKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) ILG Nurtjahjaningsih; Lukman Hakim; Yemima Viktory Utomo; Anto Rimbawanto; AYPBC Widyatmoko
Jurnal Pemuliaan Tanaman Hutan Vol 15, No 2 (2021): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2021.15.2.85-93

Timoho (Kleinhovia hospita Linn.) has high economic value for medicine and construction. However, information on genetic diversity and conservation genetic have been limited by the lack of genetic data. Acacia mangium (mangium)This study aimed to assess genetic diversity of timoho using RAPD markers. Leaf samples of seedlings were collected from a nursery at CFBTI in Yogyakarta; the seedlings originated from plantation in Gunung Kidul (GK) and arboretum in Faculty of Forestry, GMU (FK). Seven out of 22 screened RAPD markers were stable in amplification and consisted of 61 polymorphic loci; then these markers were used to analyse genetic diversity and population structure of 48 seedlings from GK and FK. The genetic diversity and structure parameters were calculated by GenAlex software. A moderate level of genetic diversity (mean uHE=0,326) and great genetic distance between the two populations (DA=0.119) were found in this species. PCoA analysis showed that the 48 individuals were divided into two clusters according to their originated population. Appropriate conservation strategies were proposed to protect genetic diversity of this species.

KARAKTER MORFOLOGI ISOLAT Phlebiopsis sp.1 JAMUR PENGENDALI HAYATI YANG POTENSIAL UNTUK Ganoderma philippii Desy Puspitasari; Arif Wibowo; Sri Rahayu; Istiana Prihatini; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Identification of wood decay fungi based on morphological character of isolates is very helpful where identification of fruit body morphology is ambiguous. Nine isolates of Phlebiopsis sp.1 obtained from the isolation root of Eucalyptus pellita in permanent plots with root rot disease have the potential as biological control for G. philippii. The verification based on ITS sequences of rDNA showed that these isolates were closely related to P. gigantea and P. flavidoalba. The characterisation of morphological was performed on isolates grown on Malt Extract Agar media with sawdust. Based on the macroscopic observation, the isolates were categorized into three different morphotypes: (1). Cottony mycelium from the beginning to the advanced stage, one isolate (Pb5); (2). Cottony at the beginning and then turned into farinaceous to floccose on the advanced stage, 7 isolates (Pb1, Pb2, Pb4, PB6, PB8, Pb9, Pb10); (3). Absent at the beginning and then turned into zonate on the advanced stage, one isolate (Pb11). Morphotype 2 is the dominant group of Phlebiopsis sp.1 isolates, with fine tufts of white mycelium which turned brown on the surface of the colonies. Morphotype 3 has very different morphological characters than other 8 isolates, it has very characteristic concentric circle with different color and texture of each circle. The growth rate of mycelium ranged between 10.70 - 10.85 cm²/day for morphotype 1 and 2; 1.95 cm²/day for morphotype 3.

STRUKTUR GENETIK POHON INDUK Calophyllum inophyllum DI TEGAKAN BENIH PROVENAN BERDASARKAN PENANDA SIMPLE SEQUENCES REPEATS ILG Nurtjahjaningsih; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 13, No 1 (2019): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

This study aimed to assess genetic structure of Calophyllum inophyllum using simple sequences repeats (SSR) markers to control genetic diversity at a provenance seed stand. Leaf samples were collected for DNA templates and 6 SSR primers were used. Genetic data of 280 trees were analyzed by FSTAT software, while PCoA was analyzed by GeneAlex. Results showed that number of allele (NA) per SSR primer between 3 and 4, the allelic richness (AR) ranged 2.805 to 4.000, the gene diversity (HE) ranged 0.018 to 0.631, coefficient inbreeding (FIS) of overall SSR primers were insignificant deviate from Hardy-Weinberg Equilibrium, excepted NY3. The mean HE values in the TBP was in low (mean HE= 0.272). However, the mean coefficient inbreeding values was not significant. PCoA analysis showed that the seed stand consisted genetically related trees; the 280 trees originated from only 5-6 mother trees. This is because the stand was originated from a conservation stand with narrow genetic differentiation. Management of the seed stand was discussed.

PENINGKATAN GENETIK AKTUAL DAN INTERAKSI SUMBER BENIH X LOKASI TERHADAP KADAR 1,8 CINEOLE DAN RENDEMEN MINYAK PADA KAYUPUTIH Noor Khomsah Kartikawati; Prastyono Prastyono; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 2 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Realized genetic gain of improved seed of cajuput from a breeding program and seed source x site interaction on the oil properties (1,8-cineole and oil yield) was evaluated through the establishment of genetic gain trials in two sites: Gunungkidul dan Ponorogo. Improved seed from two seed sources tested in the trials were first generation seedling seed orchard (SSO-1) and selected superior seed trees in the SSO-1 (SSO-2). As a control, unimproved seed from three seed sources: natural stands in Maluku (ST-1), plantation in Ponorogo (ST-2) and Gundih (ST-3) were also planted together in the trials. The genetic gain trial was established using a randomised complete clock design which consists of 5 tested seed sources, 20 treeplot, 8 replicates and spacing of 3 x 3m. The results of study showed that the realized genetic gain for the content of 1,8-cineole from SSO-2 over the controls ranged from 13.78% to 17.52% and the genetic gain of the oil yield ranged from 15.76% to 26,43%. Meanwhile the realized genetic gain for SSO-1 ranged from 2.07% to 5.42% and 10.15% to 20.30% for the content of 1,8-cineole and oil yield,respectively. The non-significance of seed source x site interaction indicates that all improved seed from the first-generation seedling seed orchard of cajuput in Paliyan are most likely to be adaptable to the varies site in Gunungkidul and Ponorogo while maintaining the high productivity in oil properties.

KEKERABATAN GENETIK ANAKAN ALAM ULIN (Eusideroxylon zwageri TEIJSM. & BINN.) MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA ILG Nurtjahjaningsih; Sukartiningsih Sukartiningsih; Anna Puspa Amarta Saranti; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 11, No 1 (2017): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

The study aimed to assess genetic diversity and genetic relationship of ulin wildlings randomly collected from a nursery and originated from Bukit Soeharto natural forest, East Kalimantan. DNA templates were extracted from leaf samples of 1.5 years old wildings. Five RAPD primers consisted 55 polymorphic loci were used for genetic studies. Genetic diversity and relationship were analyzed using GenAlex software. The results showed moderate mean value of genetic diversity (HE=0,345, SE 0,015) of the wildings. Forty eight wildings were clustered in only 3 groups; almost all wildings (65%) were clustered in one main cluster. Moreover, 4 wildlings were clones (8%). In conclusion, the 48 wildings of ulin consisted high genetic relationship and individual clones that reflects the low genetic diversity of this species.

SELEKSI DAN PEROLEHAN GENETIK PADA UJI KETURUNAN GENERASI KEDUA KAYUPUTIH (Melaleuca cajuputi subsp. cajuputi) DI GUNUNGKIDUL Sumardi Sumardi; Noor Khomsah Kartikawati; Prastyono Prastyono; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Breeding for cajuput in Indonesia has moved into advanced generation breeding cycle through establishing second-generation progeny trial. A series of selection would be practiced in the trial before converting into a seedling seed orchard to produce genetically improved seed. In this study, a series of selection, followed by prediction of genetic gain will be observed in the second-generation progeny trial of cajuput established at Gunungkidul. The trial was laid out in randomized complete block design (RCBD), 65 families, three tree-plot, four replications, and spacing of 3 × 1,5 meter. Measurement was conducted on height and diameter at 2 years age. In the trial, within-plot selection has been practiced phenotypicaly by retaining one of the best tree out of the three tree within each plot. Succesive family selection was then simulated from the result of within-plot selection. Results of study showed that estimates of heritabily for diameter were higher than that for height. Genetic and phenotypic correlation between the two traits was moderate at around 0.57. Within-plot selection practiced in the trial resulted positive selection differential for all measured traits. Diameter showed higher coefficient weight of selection (0.4280) than height (0.0406) which indicates that the practiced within-plot selection was more imposed for diameter than that for height. Genetic gain from within-plot selection calculated using selection index were 20.76% for diameter and 12.73% for height. Simulated family selection using the same coefficient weight as within-plot selection resulted lower genetic gain at around 12.26% and 7.52% for diameter and height, respectivelly.

PENGUJIAN PENANDA JENIS SPESIFIK PADA JAMUR YANG BERPOTENSI SEBAGAI AGENS PENGENDALI HAYATI PENYAKIT BUSUK AKAR PADA AKASIA Istiana Prihatini; Anto Rimbawanto; Desy Puspitasari; Dayin Fauzi
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 1 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Root rot caused by Ganoderma philippii (Bres. & Henn. ex Sacc.) Bres., is an important disease in Acacia plantation. A strategy to control this disease is currently being developed, particularly on the application of biological control agent (BCA). Species specific primers for rapid identification of potential fungi as BCA were developed. This study aimed to obtain the best DNA condition and the best primers for species specific identification. DNA with 20x dilution is the best condition for amplification of the ITS fragment thus used for rapid species identification. The best primer set to detect Cerrena sp. is CrF1/CrR1, PbF2/PbR2 is the best primer set for Phlebiopsis sp. 1. There was no specific primer suitable to detect Phlebia sp. 1 and Phlebia sp. 2 only but Pl-2F1/Pl-2R4 is the best primer set for Phlebia spp.

PENGUJIAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA UNTUK MENGETAHUI KESTABILAN GENETIK KLON JATI (Tectona grandis) ILG Nurtjahjaningsih; Toni Herawan; Reza Permatasari Rachma; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 12, No 2 (2018): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2018.12.2.127-134

This study aimed to test RAPD markers to assess genetic stability of teak clones. Two experimental steps were carried out. First, nine RAPD markers were screened to verify the level of polymorphic loci; second, the polymorphic loci were applied to test genetic stability of clones. To test polymorphism levels of the primers, DNA was isolated from eight leaf samples that were collected from a seed orchard located at Watusipat, Gunung Kidul. To verify genetic stability of clones, DNA was isolated from leaf samples of 24 ramets of 3 clones after second sub-culturing. Results showed that amplification of 5 out of 9 RAPD primers were be consisten and produced 12 polymorphic loci. The number of polymorphic alleles per locus ranged between 1 and 3; the allele sizes were between 400 and 1,050 base pairs (bps). The percentage of polymorphic loci was 100%; it meant that overall loci have high polymorphism level. Based on these loci showed that the 24 ramets are clones; there was no somaclonal variation or high genetic stability. However, these loci need to be validated using more stable DNA markers.

STRUKTUR GENETIK Calliandra calothyrsus DI INDONESIA MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA (RAPD) ILG Nurtjahjaningsih; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 10, No 1 (2016): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2016.10.1.31-38

Calliandra calothyrsus was introduced to Indonesia from Guatemala in 1930s and had been planted widely throughout Indonesia for fuel wood. Genetic diversity within and among population is an important factor for initiating breeding program. Aim in this study was to evaluate genetic structure of C. calothyrsus in Indonesia, to obtain a better understanding of the history of the distribution and efficiency of breeding strategy of this species. Leaf samples were collected in a seedling seed orchard plot from 10 populations. Six RAPD markers consisted of 34 loci were used to analyze genetic diversity and genetic structure. The results showed that genetic diversity was in low to moderate level (mean HE= 0.321). The AMOVA analysis showed that genetic differentiation among geographical sources was significant, also among populations within geographical sources and among individual within populations as well. It was revealed that the 10 populations originated from only two ancestors. The limited number of ancestors resulted low to moderate genetic distances among populations (mean Da=0.070). In conclusion, C. calothyrsus has moderate genetic diversity within population and weak genetic structure among populations. Genetic diversity in population/individual level should be considered as a selection unit in the breeding strategies.

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