GHR/HindIII Locus Polymorphisms in Intron-2 GHR Gene of Papua Local Chicken

M. A. Mu'in (Fakultas Peternakan, Universitas Papua, Jl. Gunung Salju, Amban, Manokwari 98314.)
A. G. Murwanto (Fakultas Peternakan, Universitas Papua, Jl. Gunung Salju, Amban, Manokwari 98314.)

Jurnal Sain Peternakan Indonesia
Vol 16, No 4 (2021)

This study aimed to detect single nucleotide polymorphisms (SNPs) in intron-2 on growth hormone receptor (GHR) gene in Papua local chickens using the PCR-RFLP method to study its relationship with growth characteristics. Data on the bodyweight of 49 chickens aged 1, 2, 3, and 4 months (22 males, 27 females) and DNA samples were used for this study. The DNA fragment of size 718 bp in intron-2 of the GHR gene from the study chicken was successfully amplified using a pair of specific primers. The PCR-RFLP/HindIII analysis results found this locus's two genotypes (HindIII++ and HindIII--). HindIII+ and HindIII- alleles were 0.02 and 0.98, respectively.