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All Journal Jurnal Sain Peternakan Indonesia
M. A. Mu'in
Fakultas Peternakan, Universitas Papua, Jl. Gunung Salju, Amban, Manokwari 98314.
Veterinary

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GHR/HindIII Locus Polymorphisms in Intron-2 GHR Gene of Papua Local Chicken M. A. Mu'in; A. G. Murwanto
Jurnal Sain Peternakan Indonesia Vol 16, No 4 (2021)
Publisher : Universitas Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31186/jspi.id.16.4.315-321

Abstract

This study aimed to detect single nucleotide polymorphisms (SNPs) in intron-2 on growth hormone receptor (GHR) gene in Papua local chickens using the PCR-RFLP method to study its relationship with growth characteristics. Data on the bodyweight of 49 chickens aged 1, 2, 3, and 4 months (22 males, 27 females) and DNA samples were used for this study. The DNA fragment of size 718 bp in intron-2 of the GHR gene from the study chicken was successfully amplified using a pair of specific primers. The PCR-RFLP/HindIII analysis results found this locus's two genotypes (HindIII++ and HindIII--). HindIII+ and HindIII- alleles were 0.02 and 0.98, respectively.
Co-Authors Murwanto, A. Gatot