Purifikasi Protein Fusi MBP-Mga Streptococcus pyogenes Hasil Ekspresi Heterolog di Escherichia coli

Muhaimin Muhaimin ( Jurusan Kimia-FKIP Universitas Jambi)
Oei Ban Liang ( Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, ITB)
Enny Ratnaningsih ( Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, ITB)
Endang Purwantini ( Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, ITB)
Debbie Sofie Retnoningrum ( Jurusan Farmasi, Fakultas Matematika dan Ilmu Pengetahuan Alam, ITB)

Jurnal Matematika & Sains
Vol 10, No 1 (2005)

Optimization of purification process of Streptococcus pyogenes’s MBP-Mga fusion protein from Escherichia coli pMALMga carrying mga49 gene had been carried out. The purification was conducted by using affinity chromatography column with amylose ligands. SDS-PAGE analysis gave a band at 104 kDa showing a pure MBP-Mga fusion protein.