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Oei Ban Liang
Departemen Kimia Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Teknologi Bandung
Mathematics

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Purifikasi Protein Fusi MBP-Mga Streptococcus pyogenes Hasil Ekspresi Heterolog di Escherichia coli Muhaimin Muhaimin; Oei Ban Liang; Enny Ratnaningsih; Endang Purwantini; Debbie Sofie Retnoningrum
Jurnal Matematika & Sains Vol 10, No 1 (2005)
Publisher : Institut Teknologi Bandung

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Abstract

Optimization of purification process of Streptococcus pyogenes’s MBP-Mga fusion protein from Escherichia coli pMALMga carrying mga49 gene had been carried out. The purification was conducted by using affinity chromatography column with amylose ligands. SDS-PAGE analysis gave a band at 104 kDa showing a pure MBP-Mga fusion protein.
Konstruksi Diagram Potensial-pH untuk Baja Karbon dalam Buffer Asetat secara Potensiodinamik Eksperimental Bunbun Bundjali; N. M. Surdia; Oei Ban Liang; Bambang Ariwahjoedi
Jurnal Matematika & Sains Vol 9, No 4 (2004)
Publisher : Institut Teknologi Bandung

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The pH-potential, or the so called Pourbaix, diagram for the system Fe - H2O is normally been constructed by using thermodynamic data. In this work the diagram was constructed experimentally based on potentiodynamic measurements of carbon steel in acetic acid - acetate ion buffer solutions at a temperature range of 25-85 0C. Due to the limited pH trajectory of buffer acetate solutions the resulted diagrams were not as wide pH as those of thermodynamic Pourbaix diagrams, but they are more useful for analysing carbon steel corrosion in acetate environment systems at that temperature range. In Addition the effects of pH and temperature on the primary passive potential (Epp) and Fladge potential (EF) relationship could also be obtained.
Optimasi Proses Overproduksi, Pemurnian dan Karakterisasi Protein Mga Sebagai Molekul Target Untuk Pencegahan Infeksi oleh Streptococcus Pyogenes Muhaimin Muhaimin; Oei Ban Liang; Enny Ratnaningsih; Endang Purwantini; Debbie Soefie Retnoningrum
Jurnal Matematika & Sains Vol 8, No 3 (2003)
Publisher : Institut Teknologi Bandung

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Optimization of overproduction, purification and characterization of Streptococcus pyogenes’s Mga recombinant protein from Escherichia coli pMALMga carrying mga49 gene had been carried out. The overproduction optimization was conducted by culturing the Escherichia coli pMALMga of OD600 = 0,7 in LB medium with or without glucose, and induced by 0,3 mM IPTG for a certain period of time. The protein produced was MBP-Mga fusion protein with a molecular weight of about 104 kDa. The purification was conducted by affinity chromatography using amylose ligands at pH 7,4 and continued by SDS-PAGE. One band identified at 104 kDa showed that the MBP-Mga fusion protein was pure enough for further analysis. The MBP-Mga protein was then injected into rabbit to produce polyclonal antibodies. The antibody development was monitored by dot blot and Western blot. The antibodies produced gave a positive reaction to MBP-Mga fusion protein. Adsorption and precipitation of the polyclonal antibodies which gave a positive reaction to MBP protein was done by adding pure MBP protein (56 kDa) produced by Escherichia coli pMALc2, and purified by affinity chromatography using amylose ligands at pH 7,4. The results showed that the antibodies gave a positive reaction to Mga recombinant protein and a negative reaction to MBP recombinant protein.
Co-Authors Bambang Ariwahjoedi Bunbun Bundjali DEBBIE SOEFIE RETNONINGRUM Debbie Sofie Retnoningrum Endang Purwantini Enny Ratnaningsih Muhaimin Muhaimin N. M. Surdia