Photoaffinity Labeling Study for Identification of the Target Protein of Antimalarial Isoquercitrin

Syamsurizal Syamsurizal ( Jurusan Pendidikan MIPA, FKIP Universitas Jambi)
Satoru Tamura ( Graduate School of Pharmaceutical Sciences, Osaka University, Osaka)
Mohammad Mostaqul Huq ( Graduate School of Pharmaceutical Sciences, Osaka University, Osaka)
Masafumi Kaneko ( Graduate School of Pharmaceutical Sciences, Osaka University, Osaka)
Nobutoshi Murakami ( Graduate School of Pharmaceutical Sciences, Osaka University, Osaka)

Jurnal Matematika & Sains
Vol 12, No 2 (2007)

Isoquercitrin (1), a flavonol monoglycoside showed a strong antimalarial activity. In order to identify the target protein in Plasmodium falciparum, a photoreactive isoquercitrin analog, 2-[2-[2-(biotinylaminoethoxy) ethoxy]-4-[3-(trifluoromethyl)-3H-diazirin-3-yl] benzoic acid isoquercitrinyl ester (2) has been synthesized which closely mimics the action of 1. Photoaffinity labeling of 2 revealed the apparent molecular mass of ca 70 kDa on the uninfected erythrocyte membrane.