Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Vol. 8 No. 2 (2021): December 2021

ANTIBAKTERI EKSTRAK KAPANG ENDOFIT DARI AKAR KAYU JAWA (Lannea coromandelica (Houtt.) Merr.)

Saiful Bahri (Program Studi Farmasi, Fakultas Farmasi, Institut Sains dan Teknologi National (ISTN), Jl. Moh Kahfi II, Jagakarsa, Jakarta Selatan, 12640 DKI Jakarta)
Puteri Amelia (Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas Islam Negeri (UIN) Syarif Hidayatullah, Jl. Kertamukti No.5, Cireundeu, Ciputat Timur, Kota Tangerang Selatan, 15419 Banten)
Normala Rachmawati (Program Studi Farmasi, Fakultas Farmasi, Institut Sains dan Teknologi National (ISTN), Jl. Moh Kahfi II, Jagakarsa, Jakarta Selatan, 12640 DKI Jakarta)
Aulia Fitri Firdausya (Program Studi Biologi, Fakultas Sains dan Teknologi, Universitas Islam Negeri (UIN) Syarif Hidayatullah, Jl. Ir. H. Juanda No.95 Tangerang Selatan, Banten 15412)
Firdaus Ramadhan (Laboratorium Mikrobiologi Farmasi, Institut Sains dan Teknologi (ISTN), Jl. Moh Kahfi II, Jagakarsa, Jakarta Selatan, 12640 DKI Jakarta)



Article Info

Publish Date
28 Dec 2021

Abstract

Isolation of endophytic fungi from the roots of Lannea coromandelica could be one way to know the presence of antibacterial activity that serves as a traditional medicinal remedy. This study aims to determine the presence of endophytic fungi at the root of L. coromandelica and their extract potential as an antibacterial agent. Endophytic fungi isolation was performed by direct technique and their macroscopic as well as microscopic characteristics were observed. Screening tests were conducted using methanol and ethyl acetate solvents. Antibacterial tests were conducted on Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis. This study resulted in seven isolates of endophytic fungi (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) originated from the root of L. coromandelica. RLC 5 isolate exhibited the largest diameter of inhibition activity on the screening tests and the largest diameter on the antibacterial tests. The supernatant extract of RLC 5 isolate showed the highest antibacterial activity against the test bacterium S. epidermidis. Isolasi kapang endofit dari akar Lannea coromandelica dapat menjadi salah satu cara untuk mengetahui adanya aktivitas antibakteri yang berfungsi sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui keberadaan kapang endofit pada akar L. coromandelica dan potensinya sebagai agen antibakteri. Isolasi kapang endofit dilakukan dengan teknik langsung lalu diamati karakteristik makroskopik dan mikroskopiknya. Uji penapisan dilakukan menggunakan pelarut metanol dan etil asetat. Uji antibakteri dilakukan terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus epidermidis. Penelitian ini mendapatkan tujuh isolat kapang endofit (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) yang berasal dari akar L. coromandelica. Isolat RLC 5 memperlihatkan diameter daya hambat terbesar pada uji penapisan dan diameter terbesar pada uji aktivitas antibakteri. Ekstrak supernatan isolat RLC 5 menunjukkan aktivitas antibakteri tertinggi terhadap bakteri uji S. epidermidis.

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Journal Info

Abbrev

JBBI

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology

Description

JBBI, Indonesian Journal of Biotechnology & Bioscience, is published twice annually and provide scientific publication medium for researchers, engineers, practitioners, academicians, and observers in the field related to biotechnology and bioscience. This journal accepts original papers, review ...