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Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume Puteri Amelia; Rachma Ayunda; Saiful Bahri
Jurnal Fitofarmaka Indonesia Vol 8, No 3 (2021): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33096/jffi.v8i3.729

Endophytes or endophytic fungi have been investigated as a store house of bioactive compound. This study was aimed to evaluate the potential antibacterial activity of endophytic fungi isolated from Medinilla speciosa Blume. The antibacterial test was determined by measuring the inhibition zone with disk-diffusion method. Twenty endophytes were isolated from the leaves of Medinilla speciosa Blume and identified morphologically. The results demonstrated that ten isolates showed variation in their antibacterial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633, and Shigella dysenteriae ATCC 13313. Further investigation will be needed to explore and identify the bioactive molecules of the isolated endophytic fungi.

ISOLASI DAN KARAKTERISASI BAKTERI ASAM LAKTAT DARI LIMBAH CAIR RENDAMAN KACANG KEDELAI Zakiyah Zahra Nur Amaliah; Saiful Bahri; Puteri Amelia
Jurnal Fitofarmaka Indonesia Vol 5, No 1 (2018): JURNAL FITOFARMAKA INDONESIA
Publisher : Faculty of Pharmacy, Universitas Muslim Indonesia

Tempe is an Indonesia traditional food, which in the manufacturers have a liquid waste that no use again namely soybean soaking water. Lactic Acid Bacteria (LAB) has been known at every stage of tempeh production, included at soaking soybeans. This study aimed to isolate and characterize LAB contained in soybean soaking water liquid waste. After serial dilution to 10-7, soybean soaking water liquid waste was inoculated on the MRSA contained 1% CaCO3. After incubation, there are 8 isolates which produce clear zone around their colonies with different colony morphology suspected as LAB. Morphological, physiological and biochemical characteristics were employed to identify LAB. All isolates were non-spore forming, non-motile, catalase negative, grow well at 37° and 45°C, could be able to grow in the presence of 4% and 6.5% sodium chloride. The results of safety test showed all isolates negative for hemolytic activity. Seven of eight isolates are Gram-positive, while one is a Gram-negative. But only Gram-positive were chosen as they represent the LAB characteristic. Seven isolates were identified as Lactobacillus with heterofermentative as the type fermentation. In this study, Lactobacillus casei ATCC 393 used as reference strain

Characterization of Ethanol Extract from Katumpangan Air Herbs (Peperomia pellucida L. Kunth Marissa Angelina; Puteri Amelia; Muchammad Irsyad; Lia Meilawati; Muhammad Hanafi
Biopropal Industri Vol 6, No 2 (2015)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Peperomia pellucida L. Kunth known as “Katumpangan Air” is a potential medicinal plants used traditionally for uric acid, rheumatic, headache, stomachache, antimicrobial. This study aims to establish the non-specific and specific parameters of ethanol extract from Katumpangan air (EKA). EKA was collected from three different growth places: South Tangerang (6.833%), Bogor (7.8%) and Yogyakarta (13.125%). The results show that organoleptic extract characterization from three places were same: thick, blackish green brown, bitter and have a characteristic odor. The average content of water-soluble compounds was within range 7.39-13.29%, ethanol-soluble 15.33-16.68%, flavonoid total 3.807-4.244%. The range of moisture content was 12.25-16.34%, ash total 1.21-2.78%, acid insoluble ash 0.19-1.62%, drying shrinkage 21.62-24.98%, specific gravity 1.0010-1.0034 g/mL for the non-specific parameter. Microbial contamination testing results 0.61x103-1.13x103 coloni/g, the contaminant testing mold/yeast 0.1x102-1.7x102 coloni/g, while lead metal 0.15-0.18 mg/kg, cadmium 0-0.11 mg/kg and arsenic <0.005µg/kg.

Aktivitas Antibakteri Kapang Endofit dari Tangkai Daun Tanaman Kayu Jawa (Lannea coromandelica) Saiful Bahri; Puteri Amelia; Rahayu Kusuma Ningrum; Rosario T Manalu; Firdaus Ramadhan
Al-Kauniyah: Jurnal Biologi Vol 15, No 1 (2022): AL-KAUNIYAH: JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v15i1.17973

AbstrakKapang endofit telah banyak ditemukan pada berbagai jaringan tanaman. Pemanfaatan kapang endofit dapat menjadi solusi untuk mengurangi eksploitasi tumbuhan yang berpotensi sebagai sumber senyawa bioaktif. Penelitian ini bertujuan untuk mengetahui keberadaan kapang endofit pada tangkai daun tanaman Kayu Jawa Lannea coromandelica dan potensinya sebagai agen antibakteri. Isolasi dilakukan dari tangkai tanaman Kayu Jawa dan isolat yang berhasil diperoleh dikarakterisasi secara makroskopis dan mikroskopis. Uji penapisan pada semua isolat dengan bakteri Staphylococcus aureus dan Pseudomonas aeruginosa untuk mendapatkan isolat yang berpotensi sebagai agen antibakteri. Ekstraksi isolat terpilih dilakukan dengan pelarut metanol (MeOH) dan pelarut etil asetat (EtOAc). Pengujian antibakteri menggunakan tiga bakteri uji, yaitu Staphylococcus aureus, Pseudomonas aeruginosa, dan Salmonella typhi. Hasil penelitian terdapat enam isolat yang berada di jaringan tangkai daun tanaman Kayu Jawa, yaitu PLC.1.A, PLC.1.B, PLC.2, PLC.3, PLC.4, dan PLC.5. Hasil uji penapisan menunjukkan isolat PLC.4 yang menjadi kandidat antibakteri pada penelitian ini. Fraksi MeOH memiliki aktivitas antibakteri lebih tinggi dibanding fraksi EtOAc pada semua bakteri uji dan kontrol positif (kloramfenikol) pada bakteri P. aeruginosa. Ekstrak MeOH isolat PLC.4 memiliki aktivitas antibakteri yang berpotensi sebagai sumber antibiotik baru.Abstract Endophytic fungi have been found in various plant tissues. The utilization of endophytic fungi can be a solution to reduce the exploitation of plants that are potential sources of bioactive compounds. This study aims to determine the presence of endophytic fungi on the petiole tissue of Kayu Jawa plants (Lannea coromandelica) and their potential as an antibacterial agent. Isolation and characterization of isolates was conducted macro- and microscopically. Screening test is carried out on the isolates against Staphylococcus aureus and Pseudomonas aeruginosa bacteria to obtain isolate that has the potential as an antibacterial agent. The extraction of selected isolates was carried out using methanol (MeOH) and ethyl acetate (EtOAc) solvents. Antibacterial examination used three test bacteria: Staphylococcus aureus, Pseudomonas aeruginosa, Salmonella typhi. The results showed that six isolates were found in the petiole tissue of L. coromandelica, namely, PLC.1.A, PLC.1.B, PLC.2, PLC.3, PLC.4, and PLC.5. Screening result showed that PLC.4 isolate was a candidate for antibacterial agent in this study. The MeOH fraction had higher antibacterial activity than the EtOAc fraction in all tested bacteria and from positive control (chloramphenicol) on P. aeruginosa bacteria. In conclusion, the MeOH extract of PLC.4 isolate has antibacterial activity potential as a source of new antibiotics.

ANTIBAKTERI EKSTRAK KAPANG ENDOFIT DARI AKAR KAYU JAWA (Lannea coromandelica (Houtt.) Merr.) Saiful Bahri; Puteri Amelia; Normala Rachmawati; Aulia Fitri Firdausya; Firdaus Ramadhan
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 8 No. 2 (2021): December 2021
Publisher : Balai Bioteknologi, Badan Pengkajian dan Penerapan Teknologi (BPPT)

Isolation of endophytic fungi from the roots of Lannea coromandelica could be one way to know the presence of antibacterial activity that serves as a traditional medicinal remedy. This study aims to determine the presence of endophytic fungi at the root of L. coromandelica and their extract potential as an antibacterial agent. Endophytic fungi isolation was performed by direct technique and their macroscopic as well as microscopic characteristics were observed. Screening tests were conducted using methanol and ethyl acetate solvents. Antibacterial tests were conducted on Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis. This study resulted in seven isolates of endophytic fungi (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) originated from the root of L. coromandelica. RLC 5 isolate exhibited the largest diameter of inhibition activity on the screening tests and the largest diameter on the antibacterial tests. The supernatant extract of RLC 5 isolate showed the highest antibacterial activity against the test bacterium S. epidermidis. Isolasi kapang endofit dari akar Lannea coromandelica dapat menjadi salah satu cara untuk mengetahui adanya aktivitas antibakteri yang berfungsi sebagai obat tradisional. Penelitian ini bertujuan untuk mengetahui keberadaan kapang endofit pada akar L. coromandelica dan potensinya sebagai agen antibakteri. Isolasi kapang endofit dilakukan dengan teknik langsung lalu diamati karakteristik makroskopik dan mikroskopiknya. Uji penapisan dilakukan menggunakan pelarut metanol dan etil asetat. Uji antibakteri dilakukan terhadap Escherichia coli, Bacillus subtilis, dan Staphylococcus epidermidis. Penelitian ini mendapatkan tujuh isolat kapang endofit (RLC 1A, RLC 1B, RLC 1C, RLC 2, RLC 3, RLC 4, RLC 5) yang berasal dari akar L. coromandelica. Isolat RLC 5 memperlihatkan diameter daya hambat terbesar pada uji penapisan dan diameter terbesar pada uji aktivitas antibakteri. Ekstrak supernatan isolat RLC 5 menunjukkan aktivitas antibakteri tertinggi terhadap bakteri uji S. epidermidis.

Analysis of Escherichia coli Microbial Contamination and Total Coliform Bacteria in Refill Drinking Water in Pondok Cabe Ilir Village, South Tangerang City Puteri Amelia; Ummi Habibah; Ofa Suzanti Betha
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 4, No 1 (2022)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v4i1.24699

Water is an essential nutrient for human health. It is important to maintain adequate drinking water intake to prevent dehydration, which can cause hypothermia, dizziness, constipation, and kidney stones. Currently, water-filling stations are an alternative source of drinking water because of limited access to clean water at affordable prices. The purpose of this study was to determine bacterial contamination at a drinking water station in Pondok Cabe Ilir, South Tangerang, Banten, based on Permenkes RI No. 492/Menkes/Per/IV/2010. The study was conducted using the Most Probable Number (MPN) method and the IMViC test, Triple Sugar Iron test, H2S production test, and motility test to identify Escherichia coli. The results showed that one out of five refilled drinking water samples contained Coliform bacteria above the threshold according to Minister of Health Regulation No. 492/Menkes/Per/IV/2010.

Analysis of Escherichia coli Microbial Contamination and Total Coliform Bacteria in Refill Drinking Water in Pondok Cabe Ilir Village, South Tangerang City Puteri Amelia; Ummi Habibah; Ofa Suzanti Betha
Pharmaceutical and Biomedical Sciences Journal (PBSJ) Vol 4, No 1 (2022)
Publisher : UIN Syarif Hidayatullah Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/pbsj.v4i1.24699

Water is an essential nutrient for human health. It is important to maintain adequate drinking water intake to prevent dehydration, which can cause hypothermia, dizziness, constipation, and kidney stones. Currently, water-filling stations are an alternative source of drinking water because of limited access to clean water at affordable prices. The purpose of this study was to determine bacterial contamination at a drinking water station in Pondok Cabe Ilir, South Tangerang, Banten, based on Permenkes RI No. 492/Menkes/Per/IV/2010. The study was conducted using the Most Probable Number (MPN) method and the IMViC test, Triple Sugar Iron test, H2S production test, and motility test to identify Escherichia coli. The results showed that one out of five refilled drinking water samples contained Coliform bacteria above the threshold according to Minister of Health Regulation No. 492/Menkes/Per/IV/2010.

Pelatihan Implementasi Inventarisasi Bahan Baku UMKM Nasi Pecel Ayam Bakar Narsis Menggunakan EOQ dan ROP Amelia, Puteri; Qori, Raysa Dylia; Ulfah, Mariah
Jurnal Pengabdian Manajemen Vol 4 No 02 (2025): Jurnal Pengabdian Manajemen
Publisher : Program Studi Manajemen Universitas Muhammadiyah Gresik

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30587/jpm.v4i02.9153

Micro, Small, and Medium Enterprises (MSMEs) account MSME Nasi Pecel Ayam Bakar Narsis, established in 2018, was the partner in this community service study. The aim was to optimize raw material inventory management through the application of Economic Order Quantity and Reorder Point methods. The service method used a participatory action research approach, through observation and interviews, inventory system design using EOQ and ROP. Variables such as annual demand and ordering costs were included in the calculations. The results of the service show that there is an increase in inventory capabilities, MSMEs are able to optimize the amount and time of ordering raw materials, reduce operational costs, increase production efficiency, and ensure products are always available. The ultimate outcome of this service not only provides practical solutions for the Nasi Pecel Ayam Bakar Narsis MSME but also offers an approach model that can be used by other MSMEs to manage their stock more strategically and sustainably.

Determination of Total Phenolic Content and Antioxidant Activity of Epigallocatechin Gallate Rich Fraction from Tea Leaves (Camellia sinensis L.) Amelia, Puteri; Citraningsih, Kinanti; Dewi, Asri Kusuma; Anggia, Vivi; Anggraeni, Yuni
JSFK (Jurnal Sains Farmasi & Klinis) Vol 12 No 1 (2025): J Sains Farm Klin 12(1), April 2025
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.12.1.36-40.2025

The tea plant, Camellia sinensis L., is a well-known natural product with significant health advantages. The main catechin found in green tea leaves is epigallocatechin gallate (EGCG), which has potent antioxidant properties. EGCG is a common active ingredient in products from the medical and cosmetics industries. This study examines the antioxidant properties and total phenolic content (TPC) of an EGCG-rich fraction (ERF) that is extracted from green tea leaves. TPC was quantified using the Folin-Ciocalteu method, with absorbance measured via UV-VIS spectrophotometry at 760.5 nm. Antioxidant activity was assessed using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay, with absorbance recorded at 516 nm. The findings indicated that the TPC of ERF was 437.18 mg GAE/g ± 5.54, while the IC50 value for antioxidant activity was 4.47 mg/L, This value was lower than that of the positive control, Vitamin C, which exhibited an IC₅₀ of 5.07 mg/L. These results suggest that ERF could be developed into a natural ingredient for cosmetics and dietary supplements.

Isolation of Escherichia coli bacteriophages from Domestic Wastewater in Tangerang Selatan Amelia, Puteri; Adila, Ersa Fiki; Novitri, Suci Ahda; Janatiningrum, Isra
JSFK (Jurnal Sains Farmasi & Klinis) Vol 10 No 3 (2023): J Sains Farm Klin 10(3), Desember 2023
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jsfk.10.3.368-373.2023

In recent years, there has been a continuous increase in the prevalence of antibiotic resistance among bacteria responsible for infectious diseases. Several studies have shown that the occurrence of the COVID-19 pandemic is a major factor contributing to this rapid increase. To overcome this challenge, one promising approach is the use of bacteriophages as a treatment option. Therefore, this study aims to isolate E. coli lytic bacteriophages as an alternative antibiotic for treating secondary bacterial infections of COVID-19. Isolation of bacteriophages was carried out using the plaque assay method with double layer agar technique. The isolate obtained was purified and tested for effectiveness in lysing E. coli ATCC 25922 bacterial cells. The results showed that E. coli had resistance to antibiotics, such as Amoxicillin, Clindamycin, Ciprofloxacin, Azithromycin, and Trimethoprim/sulfamethoxazole. BEB1 isolate obtained from domestic waste had activity in inhibiting the growth of the test bacteria with an effectiveness of 72% at the 25th hour. Based on these findings, it had the potential to be developed as an alternative antibiotic agent to overcome infectious diseases caused by E. coli ATCC 25922.

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