BIOVALENTIA: Biological Research Journal
Vol. 2 No. 1 (2016)

A SIMPLE PROTOCOL FOR SOMATIC EMBRYOGENESIS INDUCTION OF IN VITRO SUGARCANE ( Saccharum officinarum. L) BY 2,4-D AND BAP

Widuri, Laily Ilman (Unknown)
Dewanti, Parawita (Unknown)
Sugiharto, Bambang (Unknown)

Article Info

Publish Date
31 May 2016

Abstract

Induction of in vitro sugarcane through somatic embryogenesis technique influenced by addition of plant growth regulator. The objective of this research was to determine appropriate formulation medium for indirect somatic embryogenesis induction on two potential sugarcane SUT Event 02 and PS 881. This research carried out in three steps, callus induction, callus proliferation, and shoot regeneration. Explants taken from basal of in vitro plantlet one month SUT Event 02 and PS 881 resulted from shoot regeneration previously. Five different medium formulas applied for callus induction and one formula for proliferation and shoot regeneration. Research using completely randomized design (CRD) factorial with five different formulation induction mediums. The result showed that the best respond of indirect somatic embryogenesis on SUT Event 02 and PS 881 was medium containing  3 mgL-1of  2,4-D.

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Journal Info
BIOVALENTIA: Biological Research Journal
Website

Abbrev

biov

Publisher

Universitas Sriwijaya

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Medicine & Pharmacology

Description

BIOVALENTIA: Biological Research Journal aims to review and publish the Biological research results from researchers. The range of articles which BIOVALENTIA: Biological Research Journal publishing is the research results in Biology science and applied Biology. Biology science is included Ecology, ...