An Amperometric Biosensor For Hg(II) Based On Urease/Alginate–Chitosan Membrane Modified Screen Printed Carbon Electrode

Rochmad Kris Sanjaya (Program Studi Farmasi, Fakultas Ilmu Kesehatan, Universitas Kadiri, Kediri–Jawa Timur, Indonesia)
Nurul Ismillayli (Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mataram.)
Dhony Hermanto (Program Studi Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Mataram.)

Jurnal Pijar MIPA
Vol. 14 No. 3 (2019): September

A screen-printed three-electrode system is fabricated to prepare a novel screen-printed biosensor for rapid determination of Hg(II) in aqueous solution. The amperometric biosensor is prepared by entrapping urease in alginate–chitosan membrane to modify the screen-printed carbon electrode. The urease/alginate–chitosan membrane for Hg(II) had optimum measurement conditions at work potential of -0.15 V, pH of 7, urea concentration of 75 mM, response time of 8 seconds, inhibition time of 7 minutes and temperature of 25 °C. The resulted biosensor characteristic were found to have the range concentration of Hg(II) ion between 40-90 ppb with the detection limit I10% was 66.45 ppb, the coefficient of variance (Cv) was 0.8%, and reactivation was 5 times reuse.