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All Journal Jurnal Veteriner Biology, Medicine, & Natural Product Chemistry Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Jurnal Pijar MIPA BIO-CONS: Jurnal Biologi dan Konservasi Jurnal Bioteknologi & Biosains Indonesia (JBBI)
Rochmad Krissanjaya
Universitas Kadiri
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Education Energy Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Veterinary

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An Amperometric Biosensor For Hg(II) Based On Urease/Alginate–Chitosan Membrane Modified Screen Printed Carbon Electrode Rochmad Kris Sanjaya; Nurul Ismillayli; Dhony Hermanto
Jurnal Pijar Mipa Vol. 14 No. 3 (2019): September
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram. Jurnal Pijar MIPA colaborates with Perkumpulan Pendidik IPA Indonesia Wilayah Nusa Tenggara Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (365.062 KB) | DOI: 10.29303/jpm.v14i3.1338

Abstract

A screen-printed three-electrode system is fabricated to prepare a novel screen-printed biosensor for rapid determination of Hg(II) in aqueous solution. The amperometric biosensor is prepared by entrapping urease in alginate–chitosan membrane to modify the screen-printed carbon electrode. The urease/alginate–chitosan membrane for Hg(II) had optimum measurement conditions at work potential of -0.15 V, pH of 7, urea concentration of 75 mM, response time of 8 seconds, inhibition time of 7 minutes and temperature of 25 °C. The resulted biosensor characteristic were found to have the range concentration of Hg(II) ion between 40-90 ppb with the detection limit I10% was 66.45 ppb, the coefficient of variance (Cv) was 0.8%, and reactivation was 5 times reuse.
A Simple and Sensitive Optode Sensor Glucose Based on Immobilization Benedict Into Nata Cellulose Membranes Dhony Hermanto; Rochmad Kris Sanjaya; Nurul Ismillayli
Jurnal Pijar Mipa Vol. 15 No. 4 (2020): September
Publisher : Department of Mathematics and Science Education, Faculty of Teacher Training and Education, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.03 KB) | DOI: 10.29303/jpm.v15i4.1352

Abstract

Determination of glucose concentration in urine and blood that anyone can use at any time is Benedict reagent based chemical sensor. This optical sensor was developed by immobilizing Benedict reagent into nata cellulose as supporting material via entrapment. The nata cellulose/Benedict membrane for glucose determination has optimum condition at maximum wavelenght   of 541.57 nm, Benedict concentration of 0.4470 M, and ratio of nata cellulse mass to Benedict volume was 1:3. Characterization of optical sensor for glucose was in working range of 0-5000 ppm, limit of detection was 911,11 ppm, sensitivity was 0.0009 and reproducibility was 0.2295%.
Interleukin-1 as a Predictor Cytokine SARS-CoV: Article Review Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya; Syntia Tanu Juwita; Ester Lianawati Antoro; Ida Septika Wulansari
Biology, Medicine, & Natural Product Chemistry Vol 12, No 1 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.121.187-190

Abstract

Severe acute respiratory syndrome coronavirus (SARS-CoV) is an etiologic agent of respiratory disease that has a mortality rate of 10%. IL-1 actively participates in the inflammatory response to infection. SARS-CoV-2 appears to act on the activation and maturation of IL-1?, which in turn activates other proinflammatory cytokines, such as IL-6 and TNF-. Therefore, IL-1? is part of the cytokine storm generated by coronavirus infection. Elevated levels of the IL-1 receptor antagonist (IL-1Ra) in severe cases of COVID-19, and this marker have been associated with increased viral load, loss of lung function, lung damage, and risk of death. In addition, there is an increase in IL-1? levels in patients with severe COVID-19, and this is strongly associated with lung injury. IL-1 levels are associated with the virulence of the process, and significantly higher serum levels have been observed in severe symptomatic SARS-CoV-2 cases than in mild cases or in those infected with the 2003 SARS-CoV coronavirus or 2012 MERS coronavirus.
PENGGALAKAN KEBIASAAN HIDUP BERSIH DAN SEHAT SEBAGAI UPAYA PENCEGAHAH PENYEBARAN COVID-19 DI KELURAHAN TAMANAN, KOTA KEDIRI Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Abdimas Ilmiah Citra Bakti Vol 4 No 2 (2023)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i2.1598

Abstract

Wabah COVID-19 telah menjadi pandemi global yang memengaruhi banyak negara, termasuk Indonesia. Pemerintah dan lembaga kesehatan telah melaksanakan berbagai langkah untuk mencegah penyebaran COVID-19. Salah satu langkah penting adalah menggalakkan kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri. Pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman masyarakat tentang kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19. Metode yang digunakan dalam pengabdian masyarakat ini adalah pendekatan partisipatif dengan melibatkan masyarakat secara aktif dalam pengumpulan data dan pelaksanaan kegiatan. Kegiatan yang dilakukan meliputi penyuluhan, demonstrasi, dan penyediaan fasilitas sanitasi. Aktivitas ini diikuti oleh 50 ibu rumah tangga selama 3 bulan, terdiri dari tiga kegiatan utama penyuluhan kesehatan tentang pola hidup bersih dan sehat untuk mencegah penularan COVID-19. Data untuk penilaian kebiasaan hidup sehat menggunakan instrumen kuesioner dan checklist sebelum dan sesudah pelatihan. Pengetahuan, sikap dan psikomotor ibu rumah tangga dalam penyebaran penularan COVID-19 setelah diberikan pelatihan kebiasaan hidup sehat selama 3 bulan mengalami peningkatan. Penilaian dilakukan dengan membandingkan hasil pre-test dan post-test pelatihan peserta. Setelah mengikuti pelatihan, sebagian besar kemampuan peserta masuk kategori tinggi pada aspek pengetahuan (76%), aspek sikap (82%) dan aspek aspek psikomotor (92%). Berdasarkan hasil pengabdian ini, dapat disimpulkan bahwa penggalakan kebiasaan hidup bersih dan sehat dapat menjadi upaya efektif dalam pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri.
PENGGALAKAN KEBIASAAN HIDUP BERSIH DAN SEHAT SEBAGAI UPAYA PENCEGAHAH PENYEBARAN COVID-19 DI KELURAHAN TAMANAN, KOTA KEDIRI Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Abdimas Ilmiah Citra Bakti Vol. 4 No. 2 (2023)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i2.1598

Abstract

Wabah COVID-19 telah menjadi pandemi global yang memengaruhi banyak negara, termasuk Indonesia. Pemerintah dan lembaga kesehatan telah melaksanakan berbagai langkah untuk mencegah penyebaran COVID-19. Salah satu langkah penting adalah menggalakkan kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri. Pengabdian masyarakat ini bertujuan untuk meningkatkan pemahaman masyarakat tentang kebiasaan hidup bersih dan sehat sebagai upaya pencegahan penyebaran COVID-19. Metode yang digunakan dalam pengabdian masyarakat ini adalah pendekatan partisipatif dengan melibatkan masyarakat secara aktif dalam pengumpulan data dan pelaksanaan kegiatan. Kegiatan yang dilakukan meliputi penyuluhan, demonstrasi, dan penyediaan fasilitas sanitasi. Aktivitas ini diikuti oleh 50 ibu rumah tangga selama 3 bulan, terdiri dari tiga kegiatan utama penyuluhan kesehatan tentang pola hidup bersih dan sehat untuk mencegah penularan COVID-19. Data untuk penilaian kebiasaan hidup sehat menggunakan instrumen kuesioner dan checklist sebelum dan sesudah pelatihan. Pengetahuan, sikap dan psikomotor ibu rumah tangga dalam penyebaran penularan COVID-19 setelah diberikan pelatihan kebiasaan hidup sehat selama 3 bulan mengalami peningkatan. Penilaian dilakukan dengan membandingkan hasil pre-test dan post-test pelatihan peserta. Setelah mengikuti pelatihan, sebagian besar kemampuan peserta masuk kategori tinggi pada aspek pengetahuan (76%), aspek sikap (82%) dan aspek aspek psikomotor (92%). Berdasarkan hasil pengabdian ini, dapat disimpulkan bahwa penggalakan kebiasaan hidup bersih dan sehat dapat menjadi upaya efektif dalam pencegahan penyebaran COVID-19 di kelurahan Tamanan, Kota Kediri.
Pemberian Monosodium Glutamat Selama Masa Organogenesis Meningkatkan Perkembangan Embrio Cacat pada Mencit Lisa Savitri; Elfred Rinaldo Kasimo; Rochmad Krissanjaya
Jurnal Veteriner Vol 24 No 3 (2023)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/jveteriner.2023.24.3.328

Abstract

Monosodium glutamate (MSG) biasanya berbentuk kristal halus dan berwarna putih. Senyawa MSG dibuat melalui proses fermentasi dari bahan dasar pati (gandum) dan gula molasses (tetes tebu) yang diberi nama sebagai garam natrium dan asam glutamat. Pemberian MSG selama induk mencit bunting bersifat embriotoksik. Penelitian ini bertujuan untuk mengetahui efek toksik MSG pada perkembangan embrio selama masa organogenesis. Penelitian pemberian MSG terhadap mencit dimulai pada kebuntingan hari ketujuh yaitu pada tahap organogenesis. Perlakuan dosis MSG yang diberikan adalah dosis 528 mg/kg BB, 696 mg/kg BB, dan 872 mg/kg BB. Peubah yang diamati dalam penelitian ini adalah jumlah fetus hidup atau mati dalam rahim pada kebuntingan hari ke-18, di samping morfologi fetus, bobot fetus dan perkembangan tulang rangka fetus. Hasil penelitian menunjukkan bahwa pemberian MSG yang dilakukan secara terus menerus sejak kebuntingan hari ke-7 hingga 16 membuat MSG tersebut masuk ke dalam tubuh induk mencit yang tidak memiliki kemampuan untuk menetralisir dan mendetokfikasi senyawa-senyawa kimia sehingga terakumulasi pada embrio mencit. Zat-zat tersebut mencapai embrio melalui pembuluh darah dan memengaruhi perkembangan fetus mencit. Penelitian ini menunjukkan bahwa ada pengaruh MSG terhadap perkembangan fetus mencit (Mus musculus) selama periode organogenesis di antaranya yaitu kecacatan pada mata (mikropthalmia dan anoftalmia), hidrosefalus ringan, jumlah metakarpal, jumlah metatarsal, dan ruas cervikal vertebrae tidak sama panjang. Abnormalitas perkembangan fetus mencit selama periode organogenesis meningkat sejalan dengan peningkatan dosis MSG yang diberikan dan bervariasi antar perlakuan. Semakin tinggi dosis MSG yang diberikan pada induk mencit bunting, maka semakin sedikit jumlah fetus yang hidup. Dapat disimpulkan bahwa MSG memengaruhi perkembangan fetus mencit selama masa organogenesis.
The Correlation Between Widal Diagnostic Test, Total Leukocyte Count, and Platelet Count in Suspected Typhoid Fever Patients at RS Aura Syifa Kediri Loviana, Novi; Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.555-558

Abstract

Typhoid fever is an acute systemic infectious disease that is related to poor personal hygiene and environmental sanitation. The diagnosis of typhoid fever is established through laboratory examinations, including serological, hematological, and bacteriological tests. This research aims to determine the correlation between the Widal diagnostic test and the total leukocyte count and platelet count in suspected typhoid fever patients at RS Aura Syifa Kediri. The study design used in this research is quantitative descriptive analysis with a cross-sectional approach. The data was collected retrospectively from secondary sources, specifically medical records of patients from August 1, 2021, to March 31, 2022, at RS Aura Syifa Kediri. The total population comprised 157 patients, and a purposive sampling method was used to select 41 patients who met the inclusion and exclusion criteria. The statistical analysis using the Spearman correlation test resulted in p-values ≥ α = 0.05 for the following correlations: Widal test O with leukocytes (0.538 ≥ 0.05), Widal test H with leukocytes (0.915 ≥ 0.05), Widal test O with platelets (0.476 ≥ 0.05), and Widal test H with platelets (0.965 ≥ 0.05). These findings indicate that there is no significant correlation between the Widal test O and H with the platelet count in patients with typhoid fever. Therefore, it can be concluded that there is no significant relationship between the Widal test O and H results and the platelet count in typhoid fever patients.
An Overview of The Total Leukocyte Count in Pulmonary Tuberculosis Patients at RSUD Gambiran Kediri City Fitri, Devi Nor; Savitri, Lisa; Krissanjaya, Rochmad; Kasimo, Elfred Rinaldo
Biology, Medicine, & Natural Product Chemistry Vol 12, No 2 (2023)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2023.122.671-674

Abstract

The leukocyte count examination in tuberculosis infection serves to monitor the inflammation caused by Mycobacterium tuberculosis. An increasing leukocyte count can lead to complications such as pleuritis. The purpose of this study was to determine the total leukocyte count of pulmonary tuberculosis patients at RSUD Gambiran, Kediri City, based on gender and age. This research was a descriptive retrospective study. Data analysis was conducted using Microsoft Excel and presented in the form of tables and diagrams. The results of this study showed that pulmonary tuberculosis infection was more prevalent in males, with 26 individuals (62%) affected. In terms of age groups, the highest infection rate was in the 21-60 years age range, with 30 individuals (71%) affected. As for leukocyte count elevation (leukocytosis), it was more commonly observed in males with a count of 14.57 µL. In terms of age groups, leukocytosis was frequently observed in the 21-60 years age range, with a count of 48.66 µL. Out of the 42 samples, 19 individuals had a normal leukocyte count, while the remaining 23 individuals experienced leukocytosis. From the results of the study, it can be concluded that not all pulmonary tuberculosis patients experience leukocytosis; some patients maintain a normal leukocyte count.
Testing Antibacterial Activity of Daun Kentut (Paederia foetida L.) Leaf Extract against Escherichia coli in Vitro Savitri, Lisa; Tiyas, Eka Wahyuning; Krissanjaya, Rochmad
Biology, Medicine, & Natural Product Chemistry Vol 13, No 1 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.131.277-283

Abstract

Escherichia coli is a pathogenic bacterium that causes infectious diseases in the digestive tract and can produce Lipopolysaccharide (LPS), including lipid A. Exposure to endotoxins in lipid A, can cause systemic effects, such as sepsis, which can lead to clinical manifestations and even death. Daun Kentut (Paederia foetida L.) is one of the herbal plants containing bioactive compounds that can inhibit the growth of E. coli bacteria. The aim of this research is to determine the effective concentration of daun kentut leaf extract to inhibit E. coli growth. This study is an experimental research using maceration extraction method and antibacterial disc diffusion method with 3 repetitions at concentrations of 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%, negative control (aquadest), and positive control (ciprofloxacin) conducted from March to April 2023 in the Microbiology Laboratory of Kadiri University. The observation results of inhibition zones in each treatment varied. The presence of these inhibition zones is due to the presence of secondary metabolite compounds in daun kentut leaf extract that have antibacterial activity, such as inhibiting protein synthesis mechanisms, causing damage to cell wall permeability, and ultimately leading to bacterial cell lysis and death. The statistical analysis using the One Way Anova test with a P-value of 0.05 shows that each variable has significant differences and effects. Thus, it can be concluded that the effective extract variation is the 100% concentration because, in this study, the 100% concentration has an inhibition zone of 26.72 mm and is classified as very strong in inhibiting E. coli bacteria.
Leaf Extract of Kentut (Paederia foetida L.) as a Preventive Measure Against Interleukin-6 Expression in the Liver of Mice in a Sepsis Model Injected with Escherichia coli Savitri, Lisa; Freitas, Maria Do Carmo Da Costa; Kasimo, Elfred Rinaldo; Krissanjaya, Rochmad; Juwita, Syntia Tanu
Biology, Medicine, & Natural Product Chemistry Vol 13, No 2 (2024)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2024.132.329-336

Abstract

Kentut leaves (Paederia foetida L.) are a medicinal plant that can be used as a preventative medicine against sepsis. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. This research aims to to determine the influence and effective dosage of Kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in a sepsis model injected with E. coli. The method used was a Completely Randomized Design (CRD). The study involved 24 white male mice divided into 6 groups. Data analysis was performed using One way ANOVA. The average values of IL-6 expression in the mouse livers for each group are as follows: KN at 7.09%±0.06; K+ at 26.36%±0.02; K- at 72.60%±0.05; PI (100mg/kgBW) at 71.04%±0.04; PII (300mg/kgBW) at 62.22%±0.02; and PIII (500mg/kgBW) at 40.92%±0.01. The research results indicate an influence of kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in the sepsis model injected with E. coli, with a significance value of 0.000 or p-value < 0.005. The effective dosage of kentut leaf extract as a preventive measure against IL-6 expression is the PIII dosage of 500mg/kg BW. The anti-inflammatory mechanism in sepsis is thought to be caused by the presence of flavonoids, alkaloids, phenolic acids, and terpenoid compounds. The most likely anti-inflammatory mechanism is believed to involve flavonoids inhibiting cyclooxygenase (COX) and lipoxygenase (LOX) enzymes involved in the synthesis of inflammatory mediators such as prostaglandins and leukotrienes, which can trigger IL-6 production.
Co-Authors Alves, Silvina Sandra X. Amanda, Cornelia Antoro, Ester Lianawati Datin An Nisa Sukmawati Dhony Hermanto Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo Elfred Rinaldo Kasimo, Elfred Rinaldo Ester Lianawati Antoro Fendy Prasetyawan Fitri, Devi Nor Freitas, Maria Do Carmo Da Costa Hilmi, Mochamad Hanif Hilmi, Mochammad Hanif Ida Septika Wulansari Ihsan, Kharisul Juwita, Syntia Tanu Laili, Nur Fahma Loviana, Novi Masyhuri, Aga Adi Mebung, Konradus Klala Neni Probosiwi Nurul Ismillayli Rasyadan Taufiq Probojati Retnowati, Ana savitri, Lisa Syntia Tanu Juwita Tiyas, Eka Wahyuning Tsamrotul Ilmi Wulansari, Ida Septika Yanti, Novirma Yuneka Saristiana