Increase in alp levels at odontoblast cell line after haruan (Channa striata) extract administration as remineralization material

Juni J. Nugroho (Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia)

Journal of Dentomaxillofacial Science
Vol. 7 No. 1 (2022): (Available online: 1 April 2022)

Objective: To determine the effectivity of haruan (Channa striata) extract which added calcium hydroxide to ALP levels in odontoblast cell line.                                   Material and Methods: The study used a posttest only control group design. Odontoblast MDPC-23 cell line that has been distributed in 96-well microplates is divided into 8 groups. ALP levels were measured by ELISA reader. Data were analyzed statistically using ANOVA statistical test, followed by Bonferroni's post test.Results:The ALP level in the odontoblast cell line in haruan extract that added with calcium hydroxide increased compared to the control. At a concentration of 25 µg/ml = 208.37 µg/ml, a concentration of 50 µg/ml = 219.04 µg/ml, a concentration of 100 µg/ml = 282.93 µg/ml and differed significantly from the control group.Conclusion:The effect of haruan extract added with calcium hydroxide can increase the ALP level in odontoblast cell line as concentration increases.