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All Journal Journal of Dentomaxillofacial Science
Juni J. Nugroho
Department of Conservative Dentistry, Faculty of Dentistry Hasanuddin University, Makassar, Indonesia
Dentistry

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Enamel remineralization after extracoronal bleaching using nano-hydroxyapatite (nha) from synthesis results of blood clam (anadara granosa) shells Noor Hikmah; Juni J. Nugroho; Nurhayaty Natsir; Christine A. Rovani; Latieif Mooduto
Journal of Dentomaxillofacial Science Vol. 4 No. 1 (2019): (Available online: 1 April 2019)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.91 KB) | DOI: 10.15562/jdmfs.v4i1.691

Abstract

Objective: Nano-hydroxyapatite (nHA) is considered effective in stimulating the occurrence of enamel remineralization after application of bleaching materials. Nano-hydroxyapatite (nHA) can be synthesized from the shell of the blood clam (Anadara granosa) and the substances contained can be measured using X-ray diffraction (XRD).Material and Methods: The samples used in this study were 6 premolars of one upper jaw prepared by buccal and palatal crown with size of 5×5×2 mm (12 enamel disc), soaked in saline solution then divided into 2 treatment groups. Initial calcium percentage examinations were performed on each sample before bleaching material application. Next, bleaching material was applied to the sample according to the manufacturer's instructions and calcium level in each treatment group was re-examined. Application of CPP-ACP paste and nHA synthesis of blood clam (anadara granosa) shells was performed after bleaching with an hour application time per day for 7 consecutive days, and re-examined afterwards. The calcium percentage examination is performed using Energy Dispersive X-Ray Spectroscopy (EDS) tools. Data are collected and examined.Results: Data were collected and analyzed using independent t test. The results indicate that remineralization with nHA from synthesis results of blood clam shells increased the calcium levels of samples that had been extracoronally bleached, but statistically there was no significant difference with the value of p = 0.345 (p> 0.05)Conclusion: The nano-hydroxyapatite (nHA) application of blood clam shells (Anadara granosa) synthesis results can increase the percentage of calcium after the application of extracoronal bleaching materials.
Cytotoxicity of 5% ethanol extracts of moringa oleifera leaf as an alternative of root canal irrigant to fibroblast BHK-21 cell culture Juni J. Nugroho; Aries C. Trilaksana; Nurhayaty Natsir; Christine A. Rovani; Asrianti; Noor Hikmah
Journal of Dentomaxillofacial Science Vol. 6 No. 1 (2021): (Available online: 1 April 2021)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v6i1.1105

Abstract

Objective: The purpose of this study was to determine the cytotoxicity of 5% ethanol extracts of Moringa oleifera leaf based on the percentage of fibroblast cell life after exposure to 5% ethanol extracts of Moringa oleifera leaf.Material and Methods: This study was an experimental laboratory with posttest only control group design. The extract was macerated using ethanol. Toxicity tested using the MTT Assay method. The fibroblast cells distributed in 96 well microplate were divided into 5 treatment groups. Group I as a negative control containing culture media, group II as a positive control containing cells in culture media, group III 5% ethanol extracts of Moringa leaf, group IV 2% chlorhexidine solution and group V 2.5% NaOCl solution. The percentage of cell life is calculated from the optical density value of the ELISA reader. Data were analyzed using the Kruskal Wallis test followed by the Post Hoc test using the Mann Whitney test.Results: The results showed the percentage of life of fibroblast cells after exposure to 5% ethanol extracts of Moringa oleifera leaf = 33.31%, 2.5% NaOCl = 28.89% and 2% chlorhexidine = 39.37% (p = 0.000, p < 0.05).Conclusion: 5% ethanol extracts of Moringa oleifera and 2% chlorhexidine were moderately toxic to fibroblast cells while 2.5% NaOCl was strongly toxic (p = 0.004, p <0.05).
Increase in alp levels at odontoblast cell line after haruan (Channa striata) extract administration as remineralization material Juni J. Nugroho
Journal of Dentomaxillofacial Science Vol. 7 No. 1 (2022): (Available online: 1 April 2022)
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v5i2.1289

Abstract

Objective: To determine the effectivity of haruan (Channa striata) extract which added calcium hydroxide to ALP levels in odontoblast cell line.                                   Material and Methods: The study used a posttest only control group design. Odontoblast MDPC-23 cell line that has been distributed in 96-well microplates is divided into 8 groups. ALP levels were measured by ELISA reader. Data were analyzed statistically using ANOVA statistical test, followed by Bonferroni's post test.Results:The ALP level in the odontoblast cell line in haruan extract that added with calcium hydroxide increased compared to the control. At a concentration of 25 µg/ml = 208.37 µg/ml, a concentration of 50 µg/ml = 219.04 µg/ml, a concentration of 100 µg/ml = 282.93 µg/ml and differed significantly from the control group.Conclusion:The effect of haruan extract added with calcium hydroxide can increase the ALP level in odontoblast cell line as concentration increases.
Co-Authors Aries C. Trilaksana Asrianti Christine A. Rovani Latieif Mooduto Noor Hikmah Noor Hikmah Nurhayaty Natsir