Objective:This study was designed to investigate serum level nesfatin-1 and determine the association ofnesfatin-1, calcium level and 25-hydroxy vitamin D (25(OH )D) level in women with gestational diabetesmellitus (GDM) versus controlsMethods: A88 pregnant women at 24-28 weeks gestation were included in this study .Forty four womendiagnosed with gestational diabetes and 44 women without gestational diabetes both groups were matchedin age and body mass index . Then we classify each group depended on 25(OH) D status (sufficient,insufficient and deficient). nesfatin-1 and vitamin D levels were analyzed using ELISA (enzyme linkimmunosorbent assay) a cut off value for serum calcium were assessed using colorimetric method, cutoff value were used according to manufacture instruction and the a comparison was done between aboveparameters. Furthermore, insufficient and deficient 25(OH)D gestational diabetes group had significantlyhigher fasting blood sugar compared to matched control group .Conclusion: Nesfatin-1 levels, 25(OH)D and calcium were not differ between the two groups statistically.
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