The current work was conducted for isolation, identification and epidemiological frequency and distributionof Proteus mirabilis from humans and Dogs, cats, scanning Vitek in addition (PCR) assay was confirmdetect isolates and Api 20E technique is also used in order diagnosis of P. mirabilis then determinationof antibiotics susceptibility pattern of recovered isolates. The human isolates showed that (89.47%) wereresistant Ampicillin, (42.10%) Ofloxacin, (57.14%) Cefoxitin, (57.14%) Gentamycin, (100%) Vancomycin,(57.14%) Chloramphenicol, (89.47%) Trimethoprim, (100%) Amoxiclav, (57.89%) Streptomycin, (100%)Tetracycline, (89.47%) Penicillin, (100%) Erythromycin. The dogs isolates showed resistance (64.28%) wasresistant Ampicillin, (35.71%) Ofloxacin, (46.1%) Cefoxitin, (53.8%) Gentamycin, (64.28%) Vancomycin,(47.3%) Chloramphenicol, (64.28%) Trimethoprim, (64.28%) Amoxiclav, (42.85%) Streptomycin, (64.28%)Tetracycline, (64.28%) Penicillin, (64.28%) Erythromycin, otherwise the cat’s isolates showed resistance(35.71%) were Ampicillin -resistant, for Ofloxacin (35.71%), for Cefoxitin (35.71%), for Gentamycin(35.71%), for Vancomycin (35.71%), for Chloramphenicol (35.71%), for Trimethoprim (35.71%), forAmoxiclav (35.71%), for Streptomycin (35.71%), for Tetracycline (35.71%), for Penicillin (35.71%), forErythromycin (35.71%). The Proteus mirabilis was isolated from 33 out of 195 sample (16.92%) distributedas 19 isolated from human and 14 from Dogs and Cats. The human isolates showed that (89.47%) wereresistant Ampicillin, (42.10%) Ofloxacin, (57.14%) Cefoxitin, (57.14%) Gentamycin, (100%) Vancomycin,(57.14%) Chloramphenicol, (89.47%) Trimethoprim, (100%) Amoxiclav, (57.89%) Streptomycin, (100%)Tetracycline, (89.47%) Penicillin, (100%) Erythromycin.
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