Human conjunctival swab samples from 108 patients were male 58 (53.7 %) and female 50 (46.3 %) witha male to female ratio of 1.16:1.for the period from October 2019 to February 2020. Patients were selectedaccording to clinical diagnosis by a specialist ophthalmologist. Diagnosis was confirmed by real time PCRthat showed 84 patients (77.8%) with positive result; %).The study groups were divided into groups withdifferent age and gender. Those aged 15–40 years had much likely to be infected than those aged 55 years.Positive rate in mild cases was 8 (26.7 %), in moderate cases was 40(95.2 %) and in severe cases was 36(100.0 %). Phylogenetic tree analysis of local conjunctivitis based on the Hexon gene partial sequence ofhuman adenovirus show genetic relation to human adenovirus 8 and 56 hexon gene .
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