<![CDATA[Breast cancer is a significant illness in the world. Molecular methods are important and essential for thediagnosis of breast cancer. Many of the genes with expression modification, such as the Bcl-2 gene, areexplicitly encoding an anti-apoptotic protein and thus known as oncogene.A total of 55 patients with breastcancer divided into( 25 Stage 0 , 15 Stage IA, and15 Stage IB), and 25 control group were enrolled in thisstudy, age range of 35 – 60 years old the samples were collected from Al-Eluia hospital for woman care,oncology teaching hospital.RNA were extracted and then converted to cDNA to perform real time- PCRdone for those samples to detect the gene expression.Expression of gene in the study is higher levels inmalignant group stage IB, following stage IA ,and stage0 and the fold of expression was 10.00 time higherthan the control group, significantly higher in the third grade of breast tumor samples with Ct (22.14) of thefirst grade with CT (25.63) and the second with CT (24.07). According to the results of the study that the useof molecular methods in measuring the expression of Bcl-2 gene may help to diagnose the disease and maybe considered that the Bcl2 gene is molecular tool for the early detection of breast cancer.]]>
Copyrights © 2021
