Giardiasis infection main causative agent of humans, companion animals, livestock and wild life is G. lamblia. Thus, a total of 300 fecal samples of cattle, sheep, goats suffered from diarrhea in slaughtering house in AL-Diwanyha province were collected. The multiplex PCR technique applied for G. lamblia detection as well as genotyping. The research findings revealed that 154 positive cases, their prevalence percentage were 35, 35 and 30 respectively. Giardia lamblia E, A, B and AB assemblages genotypes showed significant differences, indicating a potential for zoonotic spread under definite situations where humans living in close contact with livestock, therefore, More awareness urgently needed to eliminate these infections.
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