USING STREPTOMYCES XYLANASE TO PRODUCE XYLOOLIGOSACHARIDE FROM CORNCOB

ANJA MERYANDINI (1. Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agriculture University, Bogor)
TITI CANDRA SUNARTI (Department of Agrotechno Industry, Faculty of Agricultural Technology, Bogor Agriculture University, Bogor, Indonesia)
APRILIA NAOMI (Department of Biology, Faculty of Mathematics and Natural Sciences, Bogor Agriculture University, Bogor, Indonesia)
FERY MUTIA (Department of Agrotechno Industry, Faculty of Agricultural Technology, Bogor Agriculture University, Bogor, Indonesia)

BIOTROPIA - The Southeast Asian Journal of Tropical Biology
Vol. 15 No. 2 (2008)

Streptomyces 234P-16 and SKK1-8 are xylanase-producing bacteria. Corncob xylan were extracted using acidified method. Crude enzymes (produced by centrifuging the culture) were used to hydrolyze xylan from 2 varieties of corncob. Crude extract activity was measured by using DNS (Dinitrosalisilic Acid) method. Xylanase from strain 234P-16 has the highest activity if cultivated in 1% Hawaii xylan, whereas strain SKK1-8 on 1.5% Bisma xylan. SKK1-8 xylanase can hydrolize corncob xylan (1% Hawaii or 1.5% Bisma xylan) within 4 hours and produce xylooligosacharide with polymerization degree of 4.76 and 6.37, respectively. Key words: Xylanase, Xylooligosacharide, Streptomyces.