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All Journal BIOTROPIA - The Southeast Asian Journal of Tropical Biology
FERY MUTIA
Department of Agrotechno Industry, Faculty of Agricultural Technology, Bogor Agriculture University, Bogor, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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USING STREPTOMYCES XYLANASE TO PRODUCE XYLOOLIGOSACHARIDE FROM CORNCOB ANJA MERYANDINI; TITI CANDRA SUNARTI; APRILIA NAOMI; FERY MUTIA
BIOTROPIA Vol. 15 No. 2 (2008)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2008.15.2.71

Abstract

Streptomyces 234P-16 and SKK1-8 are xylanase-producing bacteria. Corncob xylan were extracted using acidified method. Crude enzymes (produced by centrifuging the culture) were used to hydrolyze xylan from 2 varieties of corncob. Crude extract activity was measured by using DNS (Dinitrosalisilic Acid) method. Xylanase from strain 234P-16 has the highest activity if cultivated in 1% Hawaii xylan, whereas strain SKK1-8 on 1.5% Bisma xylan. SKK1-8 xylanase can hydrolize corncob xylan (1% Hawaii or 1.5% Bisma xylan) within 4 hours and produce xylooligosacharide with polymerization degree of 4.76 and 6.37, respectively. Key words: Xylanase, Xylooligosacharide, Streptomyces.
Co-Authors ANJA MERYANDINI APRILIA NAOMI Titi Candra Sunarti