Jurnal Medik Veteriner
Vol. 4 No. 1 (2021): April

Isolation and Identification of Protein Spike (Protein-S) in Field Isolates of Infectious Bronchitis Virus

Rahmahani, Jola (Unknown)
Pradhita, Dewanggi Kristi Sasmi (Unknown)
Widjaja, Nanik Sianita (Unknown)
Suwarno, Suwarno (Unknown)

Article Info

Publish Date
15 Apr 2021

Abstract

Infectious Bronchitis (IB) is disease causes great economic impact across nations. Spike protein (protein-S) is one of structural protein of Infectious Bronchitis Virus (IBV) located on enveloped of the virion. Molecular characterization of IBV could be conducted use Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to reveal the protein weight then confirmed through Immuno-Blotting assay. These methods were conducted to know the molecule weight of field isolate compared to seed vaccine. The results of this study indicate the weight of protein-S of field isolate I-147/11 were 168.21 kDa which was same to protein-S of seed vaccine Massachusset.

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Journal Info
Jurnal Medik Veteriner
Website

Abbrev

JMV

Publisher

Universitas Airlangga

Subject

Biochemistry, Genetics & Molecular Biology Veterinary

Description

urnal Medik Veteriner (JMV) publishes high quality and novelty papers focusing on Veterinary and Animal Science. The fields of study are anatomy, pathology, basic medicine, veterinary public health, microbiology, veterinary reproduction, parasitology, animal husbandry and animal welfare. Food ...