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All Journal Jurnal Medik Veteriner
Pradhita, Dewanggi Kristi Sasmi
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Biochemistry, Genetics & Molecular Biology Veterinary

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Isolation and Identification of Protein Spike (Protein-S) in Field Isolates of Infectious Bronchitis Virus Rahmahani, Jola; Pradhita, Dewanggi Kristi Sasmi; Widjaja, Nanik Sianita; Suwarno, Suwarno
Jurnal Medik Veteriner Vol. 4 No. 1 (2021): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol4.iss1.2021.104-109

Abstract

Infectious Bronchitis (IB) is disease causes great economic impact across nations. Spike protein (protein-S) is one of structural protein of Infectious Bronchitis Virus (IBV) located on enveloped of the virion. Molecular characterization of IBV could be conducted use Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) to reveal the protein weight then confirmed through Immuno-Blotting assay. These methods were conducted to know the molecule weight of field isolate compared to seed vaccine. The results of this study indicate the weight of protein-S of field isolate I-147/11 were 168.21 kDa which was same to protein-S of seed vaccine Massachusset.
Co-Authors Rahmahani, Jola Suwarno Suwarno Widjaja, Nanik Sianita