Molecular Detection of VEB and OXA-23 Resistance Gene in Pseudomonas Aeruginosa Isolates at Dr. Wahidin Sudirohusodo Hospital Makassar

Andi Zsazsa Rafiatul Mukhlis (Master of Biomedical Science, Graduate School Hasanuddin University)
Rizalinda Sjahril (Department of Microbiology, Faculty of Medicine, Hasanuddin University.)
Firdaus Hamid (Department of Microbiology, Faculty of Medicine, Hasanuddin University.)

Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan
Vol 16, No 1 (2022): Jurnal Profesi Medika : Jurnal Kedokteran dan Kesehatan

Pseudomonas aeruginosa is a common gram-negative pathogen in nosocomial infections in immune-compromised patients. It exhibits high rates of intrinsic resistance to many classes of antibiotics, especially beta-lactam antibiotics. Production of extended-spectrum beta-lactamase (ESBL) and genes belonging to the carbapenem-hydrolyzing class D subgroup β-lactamases (CHDL) are a problem for increasing antibiotic resistance worldwide. This study aimed to identify P. aeruginosa containing the VEB and OXA-23 genes. Eighty-five clinical isolates of P. aeruginosa from various clinical samples were identified and tested for antimicrobial susceptibility using VITEK 2 compact. VEB and OXA-23 genes were detected using the Polymerase Chain Reaction (PCR) method. The PCR results revealed that 13 (15.3%) of P. aeruginosa isolates were positive OXA-23 gene, but no isolate positive for the VEB gene in P. aeruginosa isolates. The study results demonstrated the spread of the OXA-23 gene in P. aeruginosa isolates at Dr. Wahidin Sudirohusodo Hospital Makassar.