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All Journal UNEJ e-Proceeding Nusantara Medical Science Journal Molecular and Cellular Biomedical Sciences (MCBS) Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Jurnal Ilmu Alam dan Lingkungan Indonesian Journal of Obstetrics & Gynecology Science Alami Journal (Alauddin Islamic Medical) Journal Media Kesehatan Politeknik Kesehatan Makassar Jurnal Biomedika Jurnal Riset Kesehatan Poltekkes Depkes Bandung Celebes Health Journal Indian Journal of Forensic Medicine & Toxicology JUMANTIK (Jurnal Ilmiah Penelitian Kesehatan) Majalah Anestesia & Critical Care (MACC)
Firdaus Hamid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Comparison of Helsinki CT and Rotterdam CT Scoring Systems As Prognostic Factors of Brain Injury Kenny Edward Yap; Andi Asadul Islam; Andi Ihwan; Junus Asiu Bulu Baan; Firdaus Hamid
Nusantara Medical Science Journal Volume 6 Issue 1, January - June 2021
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v6i1.13967

Abstract

Introduction: Brain injury is a major cause of death and disability. Computerized Tomography (CT) scanning of the brain is essential for diagnostic screening in need of neurosurgical intervention and also provide information about patient prognosis. Methods: This study is a retrospective study design to assess the comparison of the CT scan scoring system of the head with the prognostic factors of brain injury. Head CT Scans were assessed according to the following Rotterdam and Helsinki CT Scores. All patients were managed according to standard guidelines. Surgery was performed as indicated. The primary objective was mortality assessed using the Glasgow Outcome Scale (GOS) at discharge and 3 months after. Results: Altogether, 60 patients were included. The results of the ROC analysis of the two CT scoring systems, the Helsinki score provided a better sensitivity score> 80% compared to the Rotterdam score, with a positive predictive value of around 74% each for assessing the outcome (GOS) of brain injury patients Conclusions: The Helsinki score scoring system provides a better sensitivity compared to the Rotterdam score
Effectiveness of Giving Secang Wood Extract (Caesalpinia Sappan L) Against IL-6 And IL-10 Levels in Balb / C Mice With Vulvovaginalis Candidiasis Sri Wahyu; Firdaus Hamid; Mochammad Hatta; Khairuddin Djawad; Agussalim Bukhari; Rosdiana Natzir; Alfina Baharuddin
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14567

Abstract

The aim of this study was to test the effectiveness of secang wood extract (Caesalpinia sappan L) onIL-6 and IL-10 expression in Balb / c mice with vulvovaginalis candidiasis. This type of research is anexperimental laboratory, with a control group design using experimental animals as research objects. Theresearch subjects were BALB / c mice weighing 35-40 grams, aged 10-12 weeks, the number of experimentalanimals needed in this study was 18 for 3 random groups, each group being 6. Analysis and data processingusing the excel program SPSS Repeated ANOVA Test was used to measure differences in cytokine levels.IL-6 levels in group 1 (control) where the value of IL-6 expression after infection (H1) was 4309.40 pg /mL(IQR value 3803.8-4963.5) after treatment (H7) in the control group increased by 5578.12 pq / mL (IQR5330.3 - 6162.9). In group 2 that were given fluconazole and secang wood extract, IL-6 levels tended todecrease by 3516.4 pq / mL (IQR: 3070.4-4061.6) Secang wood after infection decreased from 3417.34 pq/ mL to 2782.97 pq / mL after treatment. In Group 2 that was given fluconazole and secang wood extract,IL-10 levels increased by 1414.8 pq / ml (IQR: 1061.7- 3158) after infection, to 3268.35 pq / ml (IQRvalue: 1789.9-3709.6) after treatment. given secang wood after infection increased from 2032.67 pq / ml to3091.83 pq / mL after treatment. The p value is 0.008. Obtained the effect of secang wood extract in reducinglevels of Interleukin 6 and can increase the value of IL-10 levels after administration of secang wood extracton candidiasis vulvovaginalis
Detection of LytA Genes in Streptococcus pneumoniae Isolated from sputum pneumonia patients Mustika Sari Hutabarat; Firdaus Hamid; Irawaty Djaharuddin; Alfian Zainuddin; Rossana Agus; Muhammad Nassrum Massi
Biomedika Vol 13 No 1 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i1.718

Abstract

Streptococcus pneumoniae (pneumococcus) is a Gram-positive facultative anaerobic bacterium that is a major cause of morbidity and mortality worldwide. But the lack of reporting of disease by this bacterium in Indonesia, one of the causes is because the diagnosis of pneumococcal infection is often clinically not typical and conventional methods which are still the standard gold method often give false-negative results. So the purpose of this study was to evaluate the performance of culture and molecular diagnostic methods using the Polymerase Chain Reaction (PCR) technique in detecting Streptococcus pneumoniae in sputum clinical samples using the Autolysin (LytA) gene which is a virulence factor of this bacterium. 57 isolates from 60 samples were confirmed as Streptococcus sp through microscopic identification, culture, and biochemical tests. Then the sensitivity test with an optochin test of 9 (9%) compared the results descriptively with the PCR technique using the Autolysin A (LytA) gene which was obtained more sensitive by 15 (25%).
Prevalence of Listeria spp. with PRS Genes in Fresh Vegetables from Traditional Markets A. Kaisar Adiwijaya Putra; Rizalinda Sjahril; Arif Santoso; Dianawaty Amiruddin; Andi Alfian Zainuddin; Firdaus Hamid
Biomedika Vol 13 No 2 (2020): Jurnal Biomedika
Publisher : Fakultas Ilmu Kesehatan Universitas Setia Budi Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31001/biomedika.v13i2.842

Abstract

Listeriosis is a foodborne infectious disease caused by Listeria monocytogenes and is considered a serious health problem, due to the severity of symptoms and a high mortality rate in worldwide This study aims to identify and determine the prevalence of Listeria species through prs gene screening of fresh vegetables distributed in several markets of Makassar City. A total of 57 fresh vegetable samples were collected from February to May 2019 in four traditional markets Makassar. The isolates is examines to phenotypically and genotypically Vitek and Multiplex PCR with prs and lmo1030 primer. Phenotype analysis did not show the presence of Listeria species, but the results of genotypic was found 8 positive prs gene samples (14.03%), consisting of 31.2% long beans, 18.2% cabbage, and 9.1% cucumber and. All Listeria species found in this study is Listeria monocytogenes. This study also provide information and additional data that prs genes can be used as screening genotype for identify Listeria species in fresh vegetables.
Nasal Karier Methicillin Resistant Staphylococcus Aureus pada Pasien IGD Rumah Sakit Universitas Hasanuddin Makassar Misriyani Misriyani; Andi Meutiah Ilhamjaya; Rizalinda Sjahril; Firdaus Hamid
Celebes Health Journal Vol 1 No 2 (2019): Oktober 2019
Publisher : Lembaga Layanan Pendidikan Tinggi (LL-DIKTI) Wilayah IX Sulawesi

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Abstract

Saat ini sulit membedakan CA-MRSA dan HA-MRSA, terutama CA-MRSA yang ditularkan di rumah sakit.Penelitian ini bertujuan mendeteksi“Nasal Karier Methicillin Resistant Staphylococcus aureus(MRSA) Pada Pasien di Instalasi Gawat Darurat Rumah Sakit Universitas Hasanuddin Makassar Periode Mei – Agustus 2017”.Penelitian ini merupakan penelitian deskriptif dan analitik dengan pendekatan cross sectional study.Pengambilan sampel swab nares anterior dilakukan selama periode Mei - Agustus2017, diidentifikasi menggunakanmetode kulturdan PCR. Dari 103 sampel yang diperoleh, 30 orang (29,13%) karier S. aureus dan 73 orang (70,87%) non karier S. aureus.Dari 30 orang yang karier S. aureus,6 orang (20,0%) MRSA dan 24 orang (80,0%) lainnya negatif MRSA (MSSA).Temuan gen spa lima kali lebih tinggi pada MSSA (84,0%) daripada temuan pada MRSA (16,0%). Sedangkan, tidak satupun (0%) gen mecC ditemukan pada MRSA maupun pada MSSA.Temuan gen pvl enam kali lebih tinggi pada MSSA (85,7%) daripada temuan pada MRSA (14,3%). Temuan gen ACME empat lebih tinggi pada MSSA (78,9%) daripada temuan pada MRSA (21,1%). Adapun distribusi temuan gen spa, mecC, pvl, dan ACME antara MRSA dan MSSA tidak berbeda bermakna secara statistik.
IDENTIFICATION OF DERMATOPHYTES BY MULTIPLEX-POLYMERASE CHAIN REACTION, POLYMERASE CHAIN REACTION-RESTRICTION FRAGMENT LENGTH POLYMORPHISM ITS1-ITS4 PRIMERS AND MVAI, AND POLYMERASE CHAIN REACTION (GACA)4 PRIMER Rizalinda Sjahril; Firdaus Hamid; Aan Yulianingsih; Novita Prastiwi; Awaluddin Awaluddin; Siska Nuryanti; Faridha Ilyas; Burhanuddin Bahar
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Laboratory identification of skin lesion is important for the correct diagnosis and choice of therapy. Microscopic examination of skin or nail scraping or hair fragments in 10%-KOH provides rapid result but fungal growth in culture is required for identification of species. Unfortunately, culture requires a few days to 2 weeks, and there is variable colony appearance and colour. Rapid and correct diagnosis has been enabled by Polymerase Chain Reaction (PCR), but has not yet been applied for routine diagnosis of patients. Therefore we investigated the ability of culture using Saboraud Dextrose Agar, multiplex-PCR, PCR-RFLP with ITS1-ITS4 primers and MvaI, and PCR with (GACA)4 primer to identify of the etiology agents of 130 patients with tinea who were positive showing hyphae in 10%-KOH preparation. Skin scrapings were collected in Makassar during January-June 2016 and examinations were carried out in the Microbiology Laboratory of Hasanuddin University. Results: Dermatophytosis occurred in 73 (56,1%) males, and 57 (43,8%) females. Scraping was obtained from 78 (60%) skin and 52 (40%) nail lesions. Based on age stratification, 68 (52,3%) were 10-18 years old, 43 (33%) were 19-45 years old, and 19 (14,6%) were >45 years old. While 39 (30%) samples grew in culture, Multiplex-PCR, PCR-RFLP with ITS1-ITS4 primers and MvaI, and PCR with GACA4 primer amplified DNA of 130 (100%), 126 (96,9%), and 106 (81,5 %) samples, respectively. Multiplex-PCR was not able to distinguish between spesies in 99 (76,2%), PCR-RFLP with ITS1-ITS4 primers and MvaI in 29 (22,3%) and PCR with GACA4 primer in 20 (15,4%) samples.
Antibiotic Susceptibility Pattern in Clinical Isolates of Pseudomonas aeruginosa Wahyunita Do Toka; Rizalinda Sjahril; Firdaus Hamid
Nusantara Medical Science Journal Volume 6 Issue 2, July - December 2021
Publisher : Faculty of Medicine, Hasanuddin University.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/nmsj.v6i2.14172

Abstract

Introduction: Pseudomonas aeruginosa is an opportunistic pathogen and causes of nosocomial infections in hospitals. Due to high antibiotic resistance and the ability to develop new resistance during antibiotic treatment, Pseudomonas aeruginosa infection is difficult to eradicate because the physical treatment becomes difficult and ineffective. This study was conducted to evaluate the antibiotic sensitivity pattern of Pseudomonas aeruginosa strains at two different hospital in Makassar. Methods: This study is a cross-sectional study from March to May 2021. The research samples were taken from the results of culture and antibiotic sensitivity tests conducted at two different hospital, Hasanuddin University Hospital and Dr. Wahidin Sudirohusodo Hospital for the period January 1 to September 30, 2019. A total of 84 samples were cultured and tested for antibiotic sensitivity of Pseudomonas aeruginosa. Results: Antibiotic sensitivity of Pseudomonas aeruginosa was best with aminoglycoside antibiotics, gentamicin (100%) at Hasanuddin University Hospital and amikacin (95.8%) at Dr. Wahidin Sudirohusodo Hospital. At Hasanuddin University Hospital followed by antibiotics amikacin (92.3%) and meropenem (84.6%). At Dr. Wahidin Sudirohusodo Hospital, Pseudomonas aeruginosa also showed good sensitivity to gentamicin (91.5%) and meropenem (77.5%). The sensitivity of Pseudomonas aeruginosa was lowest to piperacillin/tazobactam. Conclusions: This study shows that the level of effectiveness of the antibiotics meropenem, amikacin and gentamicin is high enough and it can be used as a treatment option in Pseudomonas aeruginosa infection. This study can help as a reference to prevent mortality and morbidity associated with Pseudomonas aeruginosa infection.
The Comparison of Peripheral Blood Morphology and Ferritin Level in Pregnancy Woman with Anemia Living in Lowland and Highland Areas Yulfa Rizki Amita; Eddy Hartono; Syahruni Syahrir; Firdaus Hamid; Samrichard Rambulangi; Elizabet Catherine Jusuf
Indonesian Journal of Obstetrics & Gynecology Science Volume 5 Nomor 1 Maret 2022
Publisher : Dep/SMF Obstetri & Ginekologi Fakultas Kedokteran Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/obgynia/v5n1.352

Abstract

Objective: Differences in the morphology features of red blood cells in population groups who live in the lowlands areas and the highlands areas will affect the diagnosis and appropriate therapies. Methods: The method used is a cross-sectional study of 75 pregnant women (6-38 weeks gestational age) with anemia living in highland and lowland areas found at Massenrempulu Hospital and Sitti Khadijah I Hospital. Anemia in pregnant women is diagnosed as a hemoglobin level less than 11gr/dl in the 1st and 3rd trimesters or less than 10.5 gr/dl in the 2nd trimester. The patient's blood sample was taken intravenously and performed serological and peripheral blood examination. Data were processed using the SPSS program, analyzed using univariate and multivariate tests.Results: In both results of the parametric and nonparametric test, ferritin levels showed a significant difference (p = 0.001), higher in the lowland areas group subjects (15.44 ± 36.92) compared to the highland areas group subjects (9.55 ± 30.16). There were no significant differences in morphology features (p> 0.05) between both groups.Conclusion: This study showed no significant differences in morphology features between the two groups. The lowland areas group subjects had a significantly higher ferritin level than that of the highland areas group subjects.Perbandingan Gambaran Morfologi Darah Tepi Pasien Gravid dengan Anemia Yang Tinggal di Pesisir Pantai dan Daerah PegununganAbstrakTujuan: Perbedaan ciri morfologi sel darah merah pada kelompok populasi yang tinggal di dataran rendah dan dataran tinggi akan mempengaruhi diagnosis dan terapi yang tepat.Metode: Metode yang digunakan adalah studi potong lintang pada 75 ibu hamil (usia kehamilan 6-38 minggu) dengan anemia yang tinggal di dataran tinggi dan dataran rendah yang ditemukan di RS Massenrempulu dan RS Sitti Khadijah I. Anemia pada ibu hamil didiagnosis berdasarkan kadar hemoglobin kurang dari 11gr/dl pada trimester I dan III atau kurang dari 10,5 gr/dl pada trimester II. Sampel darah pasien diambil secara intravena dan dilakukan pemeriksaan serologis dan darah tepi. Data diolah menggunakan program SPSS, dianalisis menggunakan uji univariat dan multivariat.Hasil: Pada hasil uji parametrik dan nonparametrik, kadar feritin menunjukkan perbedaan yang secara signifikan (p = 0,001), lebih tinggi pada subjek kelompok dataran rendah (15,44 ± 36,92) dibandingkan dengan subjek kelompok dataran tinggi (9,55 ± 30,16). Tidak ada perbedaan yang signifikan dalam morfologi darah tepi  (p> 0,05) antara kedua kelompok.Kesimpulan: Penelitian ini tidak menunjukkan perbedaan morfologi yang signifikan antara kedua kelompok. Subyek kelompok dataran rendah memiliki kadar feritin yang jauh lebih tinggi dibandingkan dengan subjek kelompok dataran tinggi.Kata kunci: Morfologi darah tepi, kehamilan, anemia, dataran rendah, dataran tinggi
KARAKTERISTIK PENGGUNA ANTIBIOTIK TANPA RESEP DOKTER DI KALANGAN GURU KECAMATAN LABAKKANG, KABUPATEN PANGKAJENE DAN KEPULAUAN Firdaus Hamid; Febyan Rasmin Kotto; Priady Wira Prasetia
Alami Journal (Alauddin Islamic Medical) Journal Vol 4 No 2 (2020): JULY
Publisher : Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/alami.v4i2.15157

Abstract

Sampai saat ini masih ada kesalahan pemahaman terhadap penggunaan antibiotik. Banyak antibiotik digunakan pada infeksi selain bakteri. Penggunaan antibiotik yang tidak tepat dapat menyebabkan resistensi antibiotik. Berdasarkan Kementerian Kesehatan RI angka kematian akibat resistensi antimikroba sampai pada tahun 2014 sebesar 700.000 per tahun. Resistensi dapat menyebabkan infeksi lebih sulit diobati, oleh karena itu penggunaan antibiotik harus mengikuti strategi peresepan antibiotik. Penelitian ini bertujuan untuk mengetahui gambaran tentang penggunaan antibiotik tanpa resep dokter di kalangan guru Kecamatan Labakkang, Kabupaten Pangkajene dan Kepulauan. Penelitian ini menggunakan studi cross-sectional dengan menggunakan data primer (kuesioner). Terdapat 236 sampel guru yang memenuhi kriteria inklusi dan ekslusi. 185 orang (78,4%) pernah menggunakan antibiotik tanpa resep dokter. Pengguna antibiotik tanpa resep dokter paling banyak dengan keluhan utama  demam 47 orang (25,4%), jenis antibiotik adalah golongan penisilin 113 orang (61,1%), lama pemakaian < 3 hari 132 orang (71,4%), mendapatkan informasi dari keluarga 71 orang (38,4%), tempat membeli di apotik 146 orang (78,9%), dan 161 orang (87%) menjawab bukan masalah ekonomi, alasan menggunakan antibiotik tanpa resep sebanyak 137 orang (74,1%) menjawab pengobatan terdahulu memberi hasil yang baik dan 125 orang (67,6%) menjawab tidak meminum antibiotik sampai habis, yang memiliki persediaan antibiotik sewaktu-waktu 98 orang (53%). Tingkat penggunaan antibiotik tanpa resep dokter responden cukuptinggi. Masih banyak responden yang belum memahami dengan baik kegunaan/ indikasi antibiotik yang mereka gunakan.
Serum Leptin Concentration is Correlated to Insulin Resistance in Polycystic Ovary Syndrome (PCOS) Patients Arsyi Adliah Anwar; Nusratuddin Abdullah; Andi Nursanty Padjalangi; Firdaus Hamid; Nasrudin Andi Mappeware; Efendi Lukas
Molecular and Cellular Biomedical Sciences Vol 5, No 2 (2021)
Publisher : Cell and BioPharmaceutical Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21705/mcbs.v5i2.203

Abstract

Background: Leptin resistance which leads to excessive circulating leptin levels is thought to affect ovarian function. This study aimed to study the correlation between serum leptin levels with insulin resistance in patients with polycystic ovary syndrome.Materials and Methods: This cross-sectional study was undertaken in several teaching hospitals in Makassar, Indonesia. We included patients diagnosed with polycystic ovary syndrome (PCOS) aged 18-40 years old. Serum leptin levels were examined in all eligible subjects using the enzyme-linked immunosorbent assay (ELISA) method. The results obtained were further analyzed statistically.Results: Approximately 53 PCOS subjects were included in this study, 25 subjects with insulin resistance and 28 subjects without insulin resistance. After examining serum leptin levels, we found that leptin is directly proportional to insulin resistance (p<0.001). We even found a strong positive correlation between serum leptin levels with homeostatic model assessment for insulin resistance (HOMA-IR) levels (r=0.659; p<0.001). Leptin was found to be independent of HOMA-IR, not influenced by confounding factors such as body mass index (BMI) (p=0.090).Conclusion: There was a significant correlation between serum leptin levels and HOMA-IR values in PCOS patients. This correlation was found to be significant regardless of patient's BMI, therefore is considered to have a direct effect on insulin resistance in PCOS.Keywords: polycystic ovary syndrome, leptin, insulin resistance, HOMA-IR
Co-Authors A. Kaisar Adiwijaya Putra A.M. Takdir Musba Aan Yulianingsih Aan Yulianingsih Anwar Ade Rifka Junita Agussalim Bukhari Ahyar Ahmad Ahyar Ahmad Alamsyah Ambo Ala Husain Alfian Zainuddin Alfina Baharuddin Aminuddin Aminuddin Andi Alfian Zainuddin Andi Alfian Zainuddin Andi Asadul Islam Andi Ihwan Andi Meutiah Ilhamjaya Andi Nursanty Padjalangi Andi Rofian Sultan Andi Zsazsa Rafiatul Mukhlis Arif Santoso Arif Santoso Arsyi Adliah Anwar Awaluddin Awaluddin Baedah Madjid Besse Afriana Arif Bukhari, Agussalim Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Burhanuddin Bahar Dario A Nelwan Dianawaty Amiruddin Eddy Hartono Eddyman W Ferial Efendi Lukas Elizabeth Catherine Jusuf Erwin Chandra Evi Damayanti Faridha Ilyas Fatmawati Annisa Syamsuddin Febyan Rasmin Kotto Fonny Josh Hardjono Sumarlie Hursepuny, Valentine Irawati Djaharuddin Irawaty Djaharuddin Junus Asiu Bulu Baan Kenny Edward Yap Khairuddin Djawad Lisa Tenriesa M. Sabir Madonna Damayanthie Datu Misriyani Misriyani Mochammad Hatta Mochammad Hatta Mochammad Hatta Muh. Nasrum Massi Muh. Ramli Ahmad Muhammad Nassrum Massi Muhammad Reza Primaguna Mustika Hutabarat Sari Mustika Sari Hutabarat Nasrudin Andi Mappeware Nita Mariana Noor Ramadhaniah Novita Prastiwi Nur Indah Purnamasari Nur Surya Wirawan Nusratuddin Abdullah Priady Wira Prasetia Ressy Dwiyanti Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rizalinda Sjahril Rosdiana Natzir Rossana Agus Sachraswaty Rachman Samrichard Rambulangi Sharvianty Arifuddin Siska Nuryanti Siti Fitrah Irmiyanti Hamid Solly Aryza Sri Wahyu Sri Wahyuni Sulmiati Sulmiati Suryani As’ad Syahruni Syahrir TOKA, WAHYUNITA DO Veni Hadju Wahyuni Alinengsih Yoeke Dewi Rasita Yulfa Rizki Amita