Indonesian Journal of Biotechnology
Vol 27, No 2 (2022)

Effects of different parameters on cellulase production by Trichoderma harzianum TF2 using solid‐state fermentation (SSF)

Jeffrey Lim Seng Heng (Biological Control Program, Agrobiodiversity and Environmental Research Centre, Malaysia Agriculture Research and Development Institute, Persiaran MARDI‐UPM, Serdang 43400, Malaysia)
Halizah Hamzah (Biological Control Program, Agrobiodiversity and Environmental Research Centre, Malaysia Agriculture Research and Development Institute, Persiaran MARDI‐UPM, Serdang 43400, Malaysia)



Article Info

Publish Date
29 Jun 2022

Abstract

Solid‐state fermentation is one of the easiest and cheapest methods for producing microbial bioactive com‐ pounds. Trichoderma harzianum has long been recognised as one of the potential fungi for this purpose. Trichoderma sp. were isolated from banana rhizosphere using the soil dilution method and later screened for their ability to produce cellulases using filter paper activity (FPase) and the carboxylmethyl cellulase (CMCase) test. Trichoderma sp. were also subjected to one factor change at a time to determine the effects of different parameters on cellulase production. It was observed that T. harzianum TF2 showed the ability to produce higher cellulase activity when wheat bran was used as the substrate. The results showed that 38.5 U/g of cellulase was produced with the use of wheat bran coupled with an incubation temperature of 28 °C and moisture content of 60%. T. harzianum TF2 showed good potential for use as a culture for cellulase production in this study due to its higher cellulase production under solid‐state fermentation, with the possibility of its application to industry.

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Journal Info

Abbrev

ijbiotech

Publisher

Subject

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

Description

The Indonesian Journal of Biotechnology (IJBiotech) is an open access, peer-reviewed, multidisciplinary journal dedicated to the publication of novel research in all aspects of biotechnology, with particular attention paid to the exploration and development of natural products derived from ...