Al-Kimia
Vol 10 No 1 (2022): JUNI

Production, Purification and Characterization of Cellulase from Cossus cossus Larvae

Maswati Baharuddin (UIN Alauddin Makassar)

Article Info

Publish Date
27 Jun 2022

Abstract

This study aimed to purify and characterize the biochemical properties of cellulase from bacterial isolates of C. cossus larvae, to determine the effect of adding crude extract cellulase and pure enzymes to the hydrolysis of rice straw. The research steps included purification of enzymes by ion exchange chromatography and gel filtration chromotography, as well as SDS-PAGE electrophoresis analysis. Pure cellulases are characterized by their biochemical properties and applied in the hydrolysis of cellulose. The results showed that the characteristics of an optimum pH of 5.6, an optimum temperature of 70⁰C and a substrate concentration of 1.5% CMC, and a molecular weight of 36.49 kDa.

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Journal Info
Al-Kimia
Website

Abbrev

al-kimia

Publisher

Universitas Islam Negeri Alauddin Makassar

Subject

Chemistry

Description

Al-Kimia is a journal of chemistry that published by Department of Chemistry, Faculty of Science and Technology UIN Alauddin Makassar. The journal is published twice a year on January-June and July-December. This journal encompasses original research articles in all aspects of chemistry and related ...