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ANALISIS PERBEDAAN KANDUNGAN LIPIDA MIKROALGA (Tetraselmis chuii dan Nannochloropsis oculata) PADA AIR LAUT DAN AIR PAYAU Baharuddin, Maswati
Teknosains Vol 5, No 1 (2011): JANUARI
Publisher : Fakultas Sains dan Teknologi UIN ALauddin

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Abstract

This research quality eksperiment with different extracted of lipid microalgae Tetraselmis chuii dan Nannochloropsis oculata from two places from sea water and  brachis water  with  pH and salinity to be different. Culture to do for eigh day just process extracted to do for ± three day. Extraction of lipid that had been done using chloroform : methanol (2:1) . product research can to be different  growth and pregnancy womb content of lipid  that can to do with use sea water media and brachis water media. The result of the research have found that % total lipid will obtained more when growing by using brackish water medias that is obtained counted 15,9 % for the Tetraselmis of chuii and 17,51% for the Nannochloropsis of oculata, while if using sea-water media only obtained 14,8 % and 16,3%. This matter is influenced by some factors like temperature, light intensity, and salinitas of pH.
PRODUKSI BIOETANOL DARI JERAMI PADI (Oryza sativa L.) dan KULIT POHON DAO (Dracontamelon) MELALUI PROSES SAKARIFIKASI DAN FERMENTASI SERENTAK (SFS) Baharuddin, Maswati; Sappewali, Sappewali; Karisma, Karisma; Fitriyani, Jeni
Chimica et Natura Acta Vol 4, No 1 (2016)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.44 KB) | DOI: 10.24198/cna.v4.n1.10441

Abstract

Bioetanol merupakan energi alternatif yang dapat diproduksi dari biomassa seperti jerami padi dan biomassa berlignoselulosa. Biomassa  lignoselulosa  tersedia terus menerus dalam jumlah yang banyak, tetapi hanya dimanfaatkan sebagai pakan ternak, dan kadang-kadang sisanya dibakar. Jerami padi dan pohon dao memiliki komponen selulosa, hemiselulosa dan lignin. Penelitian ini bertujuan untuk mengetahui kadar glukosa yang dihasilkan  melalui proses hidrolisis jerami padi dan pohon dao menggunakan enzim selulase serta menentukan kadar etanol yang dihasilkan dari proses fermentasi glukosa menggunakan Saccharomyces cerevisiae. Proses sakarifikasi dan fermentasi serentak (SFS) dilakukan dalam reaktor yang sama. Hasil penelitian menunjukkan bahwa fermentasi selama 3, 5, 7 dan 9 hari dihasilkan kadar dan konsentrasi bioetanol yang berbeda. Dari penelitian yang dilakukan kadar glukosa jerami padi adalah 105 mg/L dan dari  kulit pohon dao  sebanyak 216 mg/L.Volume bioetanol tertinggi pada hari ke-7 dengan tingkat kemurnian tertinggi pada pohon dao 0,97% pada jerami padi dengan 0,24%.
Pengaruh Penambahan Ammonium Sulfat Terhadap Kadar Serat dan Ketebalan pada Nata de Soya dari Limbah Cair Tahu Ismawanti Ismawanti; Maswati Baharuddin; Wahyu Rizandi
Al-Kimia Vol 1 No 1 (2013): June
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (298.801 KB) | DOI: 10.24252/al-kimia.v1i1.1574

Abstract

The research aimed to know the production of cellulose in tofu liquid waste, the effect of adding ammonium sulfate to the nata de soya, and the quality nata de soya produced from Tofu Liquid Waste. The parameters used are the determination of the thickness and weight, measure of water, and fiber test of nata de soya. By using variations of the addition of ammonium sulfate 10 g, 15 g, 20 g, and 25 g. 35 grams of sugar in 500 mL of tofu liquid waste, starter bacteria Acetobacter xylinum as much 100 mL, and fermented for 14 days at a temperature of 28-30oC. The quality nata de soya highest of the addition 15 grams of ammonium sulfate with a thickness of 1.7 cm, weight 231.0 grams, the lowest water content of 94.23% and the highest fiber content of 2.41%.
Isolasi dan Uji Aktivitas Enzim Selulase dari Bakteri Simbion Larva Kupu-Kupu Family: Cossidae Terhadap Variasi Lama Inkubasi Rina Dwismar; Maswati Baharuddin; Syamsidar HS
Al-Kimia Vol 1 No 1 (2013): June
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.473 KB) | DOI: 10.24252/al-kimia.v1i1.1623

Abstract

The study is titled Isolation and Testing Cellulase Enzymes from Bacterial Symbiont butterfly larvae Family: Cossidae of Variations on Old Incubation aimed to isolate cellulase enzymes in larvae of butterflies and determine tIsolasi dan Uji Aktivitas Enzim Selulase dari Bakteri Simbion Larva Kupu-Kupu Family: Cossihe optimum incubation time of enzyme production and activity of enzymes to degrade CMC substrate. In this study, an enzyme produced from the microbial gut symbionts Cossidae butterfly larvae that had been cultured in media cellulolytic, then produced the long incubation variation of 0, 12, 24, 36, 48, 60, and 72 hours, crude enzyme obtained by centrifugation at a speed of 5000 rpm at 40C and then tested enzyme activity using UV-VIS spectrophotometer. From the research result that cellulolytic bacteria can be isolated from the gut of larvae of butterlies Family: Cossidae with old optimum activity of cellulase enzyme production is 60 hours with a value of activity amounted to 2,38 x 10-3 U/mL.
Pengujian Aktivitas Antibakteri Minyak Atsiri Jahe (Zingiber Officinale Roscoe) Terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli Sufriyana Ali; Maswati Baharuddin; Sappewali Sappewali
Al-Kimia Vol 1 No 2 (2013): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (347.992 KB) | DOI: 10.24252/al-kimia.v1i2.1629

Abstract

Testing the antibacterial activity of the Essential Oil of Ginger (Zingiber officinale Roscoe) against bacteria Staphylococcus aureus and Escherichia coli. Purpose of this study is to determine the bacterial growht and activity test active compounds in ginger essential oils that can be used as an antibacterial to inhibit the growth of bacteria. This research uses a method soxhletation with solvent n- hexane to obtain the essential oil of ginger, making the media NA, rejuvenation bacteria, the manufacture of bacterial inoculum and suspension test. Antibacterial activity test using paper disc diffusion method in the form and method of dilution and analysis using GC-MS instrument. Antibacterial activity test result using paper disc method with a concentration of 25%,  50%, 75% and 100% in S. aureus (12, 34; 19, 42; 17, 34; 21, 7) mm and E. coli (10, 56; 13, 76; 16, 5; 23, 6) mm, while the result of the minimum inhibitory concentration (MIC) using ginger essential oil dilution method in S. aureus not found and E. coli at a concentration of 100%. The results of the analysis of essential oil components by GC-MS showed the active compounds in the form of compounds containing hydroxyl groups such as nerol, β- eusdesmol, borneol and compounds containing phenolic groups like zingerone.
Isolasi Senyawa Aktif Ekstrak Etanol Biji Alpukat (Persea americana) dan Uji Toksisitas Terhadap Artemia Salina Leach Andi Nur Fitriani Abubakar; Aisyah Aisyah; Maswati Baharuddin
Al-Kimia Vol 2 No 1 (2014): June
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (457.686 KB) | DOI: 10.24252/al-kimia.v2i1.1635

Abstract

Avocado seed (Persea americana) is recognized as one of medicinal plants. It contains several secondary metabolites, which have toxic activity. However, efforts to identify active compounds from avocado seeds (Persea americana) are still relatively rare. Therefore, isolation and toxicity assay have been conducted foward the active compound of avocado seed. Maceration one kilogram of seed dried powder by ethanol obtained 49,7464 gram extract. Separation of etanol extract by column chromatography generated 0,0698 grams of pure white needle crytal, which is positively triterpenoid based on Lieberman-Buchard test. In addition, infrared spectrum showed the existence of OH, C=C, C-C, C=O, -C-H, -CH3,-CH2 and C-O stretch, which support the presumed compound. The result of toxicity test on Artemia salina Leach showed that the extract, fraction and pure isolates of the etanol extract are toxic with LC50 values 13,274 g/mL; 9,528 g/mL and 8,128 g/mL, respectively.
Identifikasi Metabolit Sekunder Ekstrak Etil Asetat Biji Alpukat (Persea americana Mill.) dan Uji Toksisitas Terhadap Larva Udang Artemia salina Leach. Musyarrifah Musyarrifah; Asriani Ilyas; Maswati Baharuddin
Al-Kimia Vol 2 No 1 (2014): June
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (414.451 KB) | DOI: 10.24252/al-kimia.v2i1.1637

Abstract

Since ancient times the people of Indonesia have known, and using nutritious plant as one of the efforts in the prevention of health problems. Plants belonging to the family Lauraceae is one of a group of plants commonly used by communities as traditional medicine, one of which is Avocado (Persea americana Mill.) Especially in the seeds, which contain secondary metabolites, namely polyphenols, tannins, flavonoids, triterpenoids, quinone, monoterpenoid and seskuiterpenoid and saponins. This study aims to identify the secondary metabolites contained in the seeds of avocado (Persea americana Mill.) And determine the toxicity of extracts and pure compounds using Brine Shrimp Lethality Test (BSLT). Avocado seed solvent extracted using ethyl acetate and the extract obtained was tested group and toxicity testing. The results showed that the ethyl acetate extract of avocado seeds contain phytochemicals based on test results of  secondary metabolites, the flavonoids, alkaloids and steroids. Avocado seed solvent extracted using ethyl acetate and the extract obtained was tested group and toxicity testing. The results showed that the ethyl acetate extract of avocado seeds contain phytochemicals based on test results of secondary metabolites, the flavonoids, alkaloids and steroids.
Identifikasi Metabolit Sekunder Ekstrak Etil Asetat Biji Alpukat (Persea americana Mill.) dan Uji Toksisitas Terhadap Larva Udang Artemia salina Leach. Nurdia Asdar; Asriani Ilyas; Maswati Baharuddin
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1000.826 KB) | DOI: 10.24252/al-kimia.v2i2.1643

Abstract

Identification of metabolite compound in acetone Extract from Fruits Avocado seed (Percea americana Mill) and toxicity test against Artemia salina Leach. Aim of this research is to identify the secondary metabolite compound in aceton extract from fruits Avocado seed (Percea americana Mill) and to determine the potential of secondary metabolites from an avocado extract as anticancer. The compound was obtained from Identification process in several stages, namely extraction, fractination, purification and identification. The identification process was color test, TLC, spectroscopy IR. The toxicity test by Brine Shrimp Lethality Test (BSLT) to Artemia salina Leach. The compound obtained from this research was yellow-white crystalline needle-shaped, the purity test with TLC analysis showed a stain in three eluen system on the stain test on three eluent systems with Rf proportions as follows; 0,15 from chloroform:ethyl acetat (6:4), 0,33 from methanol:chloroform (1:9), 0,75 for acetone:ethyl acetat, and positively to the reagent Wagner and gave brown precipitate for indicate as alcaloid group. This result is supported by spectroscopy from compound.While toxicity tests showed condensed acetone extracts  and pure compounds are toxic with LC50 value of each is 20.61 mg/mL and 39,81 mg/mL.
Isolasi Dan Karakterisasi Bakteri Simbion Larva Kupu-Kupu Cossus cossus Penghasil Enzim Selulase Maswati Baharuddin; Abd. Rauf Patong; Ahyar Ahmad; Nursiah La Nafie
Al-Kimia Vol 2 No 2 (2014): Desember
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.729 KB) | DOI: 10.24252/al-kimia.v2i2.1653

Abstract

This study was conducted to characterize bacterial symbionts of butterfly larvae Cossus cossus capable of degrading cellulose. This study successfully purify and characterize isolates originating  from the intestine (CC1 and CC2), head (CC3), middle (CC4), and tail (CC5). From a qualitative test using 0.1% congo red gained the clear zone indicates that the bacteria are able to degrade cellulose. Based on the test temperature and pH on the growth of the data obtained CC5 isolates including isolates of thermophilic bacteria, while others including mesophilic bacteria. While based pH test all isolates were able to grow well at neutral pH. Based on the data obtained growth curve maximum bacterial growth at the 24th hour. Based on morphology and physiology test obtained bacteria genus Acinotobacter, Pseudomonas, and Bacillus.
Produksi dan Uji Aktivitas Enzim Selulase dari Bakteri Bacillus subtilis Al Maratun Sholihati; Maswati Baharuddin; Santi Santi
Al-Kimia Vol 3 No 2 (2015): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (368.46 KB) | DOI: 10.24252/al-kimia.v3i2.1672

Abstract

Enzyme cellulose can be produced from bacteria Bacillus subtilis. This bacteria is a kind of bacteria which grounded on the genus that can degrade cellulose to glucose. Cellulase enzyme acquired different optimum pH and temperature depends on the bacteria. This research aim to produce and analyze the activity of the cellulose enzyme from bacteria Bacillus subtilis at optimum pH and temperature. The research of this enzyme cellulose derived by prolific process and rejuvenation  bacteria Bacillus subtilis on the nutrition culture, nutrition selective, and nutrition production that executed by cold centrifugation to obtain extract or the enzyme cellulose at the temperature of 4°C, 3500 rpm within 15 minutes. On the determine pH with variety pH 5,6 using buffer acetat and pH 6,0; 6,5; 7,0; 7,5; and 8.0 using phosphate buffer while on the determine temperature using variety temperature 25°C, 30°C, 35°C, 40°C, 45°C, and 50°C which the next make an experiment activity enzyme cellulose by method Nelson-Somogy that measured in spectrophotometer UV-Vis of λ 540 nm. The result shows that the highest enzyme activity at optimum pH of 6, 0 is 4,3661 10ˉ3 U/mL and in optimum temperature of 30°C as is 5,6609 10ˉ3 U/mL.