IFN-γ and IL-2 Secretion after ESAT-6-CFP-10 (EC-610) Fusion Antigen Stimulation from Patients with Active Lung Tuberculosis and Latent Lung Tuberculosis

Bastian (Department of Biomedical, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)
Nova Kurniati (Division of Allergy and Clinical Immunology, Department of Internal Medicine, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)
Kemas Ya'kub Rahadiyanto (Department of Clinical Pathology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)
M.Irsan Saleh (Department of Pharmacology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)
Zen Hafy (Department Histology, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)
Fransisca Srioetami Tanoerahardjo (Molecular and Microbiology Laboratory, TB Research Center for Biomedical and Basic Health Technology, National Center for Health Research and Development, Ministry of Health Republic of Indonesia, Jakarta)
Jusak Nugraha (Division Immunology, Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia)
Eddy Mart Salim (Division of Allergy and Clinical Immunology, Department of Internal Medicine, Faculty of Medicine, Universitas Sriwijaya, Palembang, Indonesia)

Biomedical Journal of Indonesia
Vol. 6 No. 2 (2020): Vol 6, No 2, 2020

The Secretion of IFN-γ and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Active and Latent TB Patients. This study held to discover how immune responses work and to know the pathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells with ESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-γ and IL-2 with ELISA antibody sandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube with QuantiFERON for IFN-γ assay. The other four tubes were PBMC isolated using Ficoll-Paque, and pre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C and measured using T-Spot and ELISA reader. We got from this study that there are no significant differences in IFN-γ levels for both groups with active TB and latent TB. Measurement of IL-2 levels showed significant differences between the two group.