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All Journal Folia Medica Indonesiana Biomedical Journal of Indonesia Jurnal SainHealth Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indian Journal of Forensic Medicine & Toxicology Jurnal Widya Medika
Jusak Nugraha
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Biochemistry, Genetics & Molecular Biology Chemistry Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Other

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Sekresi IFN-γ dan IL-10 Setelah Stimulasi Antigen Fusi ESAT-6-CFP-10 (EC610) pada Penderita TB Aktif dan TB Laten Sabrina Prihantika; Nova Kurniati; Kemas Ya’kub Rahadiyanto; M. Irsan Saleh; Zen Hafy; Francisca Srioetami Tanoerahardjo; Jusak Nugraha; Eddy Mart Salim
Biomedical Journal of Indonesia Vol. 5 No. 3 (2019): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Abstract

Sistem imunitas seluler sangat berperan dalam melawan infeksi TB yaitu peran sel limfosit T yang berdiferensiasimenjadi sel Th1 mensekresikan sitokin pro inflamasi IFN-γ dan sel Th2 yang mensekresi sitokin anti inflamasi IL-10.Antigen fusi EC610 bersifat spesifik dan memiliki antigenitas yang kuat terhadap stimulasi sel T. Tujuan penelitian untukmengetahui perbedaan rerata kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610 pada penderita TB aktif dan TBlaten. Desain penelitian ini adalah eksperimen semu secara in vitro dengan kultur PBMC yang distimulasi oleh antigenEC610 pada kelompok TB aktif dan TB laten. Penelitian dan pemeriksaan dilakukan di RSK Paru Provinsi Sumsel danLaboratorium Pusat Penelitian dan Pengembangan Kesehatan, Jakarta Pusat. Terdapat 21 subjek penderita TB aktif dan28 subjek penderita TB laten yang sesuai dengan kriteria inklusi. Pengukuran kadar IFN-γ dan IL-10 dilakukanmenggunakan ELISA-Reader. Hasil penelitian menunjukkan kadar IFN-γ dan IL-10 setelah stimulasi antigen EC610lebih tinggi pada TB aktif daripada TB laten. Tingginya kadar IFN-γ pada penderita TB aktif menunjukkan adanyarespon imun protektif terhadap kuman M.tb sedangkan tingginya kadar IL-10 menunjukkan perannya sebagai antiinflamasi . Tidak terdapat perbedaan bermakna rerata kadar IFN-γ pada penderita TB aktif dan TB laten (p=0,769) danterdapat perbedaan bermakna reata kadar IL-10 setelah stimulasi antigen EC610 lebih tinggi pada TB aktif daripada TBlaten (p=0,000).
IFN-γ and IL-2 Secretion after ESAT-6-CFP-10 (EC-610) Fusion Antigen Stimulation from Patients with Active Lung Tuberculosis and Latent Lung Tuberculosis Bastian; Nova Kurniati; Kemas Ya’kub Rahadiyanto; M. Irsan Saleh; Zen Hafy; Francisca Srioetami Tanoerahardjo; Jusak Nugraha; Eddy Mart Salim
Biomedical Journal of Indonesia Vol. 6 No. 2 (2020): Biomedical Journal of Indonesia
Publisher : Fakultas Kedokteran Universitas Sriwijaya (Faculty of Medicine, Universitas Sriwijaya) Indonesia

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Abstract

The Secretion of IFN-γ and IL-2 After ESAT-6-CFP-10 Fusion Antigen Stimulation in Activeand Latent TB Patients. This study held to discover how immune responses work and to know thepathogenesis of active TB and latent TB patients. This study used PBMC to stimulate T Cells withESAT-6 and CFP-10 antigen fusion, and measure the level of IFN-γ and IL-2 with ELISA antibodysandwich (U-Cytech). 16 ml of blood were drawn to 5 tubes. ESAT-6 CFP-20 inducted one tube withQuantiFERON for IFN-γ assay. The other four tubes were PBMC isolated using Ficoll-Paque, andpre-incubated with stimulation of ESAT-6 CFP-10 fusion antigen for 24-72 hours at 370 C andmeasured using T-Spot and ELISA reader. We got from this study that there are no significantdifferences in IFN-γ levels for both groups with active TB and latent TB. Measurement of IL-2 levelsshowed significant differences between the two group.
The Concordance of Dysmorphic Erythrocyte and Cast Erythrocyte Examination using Flowcitometry, Low Condenser Light Microscope, and Phase Contrast Microscope in Children with Glomerular Hematuria Maya E. Roring; Jusak Nugraha; Risky V. Prasetyo; Ferdy R. Marpaung
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.16964

Abstract

Homesickness, Anxiety and Depression among Pakistani International Students in Indonesia during Covid-19 Outbreak Shahzad Shoukat; Cyuzuzo Callixte; Theresia Indah Budhy; Jusak Nugraha; Tuyishimire Irene
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17077

Abstract

Introduction: International students are exposed to multiple mental health crisis due to social, environmentaland culture shocks in foreign lands but it gets worse in presence of life- threatening disease outbreak. Thisresearch was conducted to evaluate the homesickness, anxiety and depression among Pakistani internationalstudents in Indonesia during Covid-19 Outbreak. Methods: This study was conducted on 86 randomstudents that are pursuing their studies in different public and private universities in Indonesia. The datawere collected by distributing google forms via email and social media groups and the responses fromthe respondents were recorded and analyzed statistically. Results: The study findings revealed that thereis a correlation between Covid-19 pandemic and the development of various mental health crisis where33 students (38.37 %) developed homesickness, 11 students (12.79%) developed anxiety and 2 students(2.33 %) manifested low-grade depression. Homesickness was outstandingly observed in females (72.72%) whereas anxiety was highly reported in males (90.90%).The study findings demonstrated that there isa strong negative correlation between student financial statuses and the development of homesickness (r:-0.977, P: 0.023) and anxiety (r: -0.944, P: 0.056).Conclusion: All in all, the fear to contract coronavirus,lockdowns, financial instability, death toll of citizens and medical professionals, run out of medical facilitiesand social media hoaxes are significant risk factors of mental health crisis among Pakistani internationalstudents in Indonesia.
Correlation Luteinizing Hormone (LH)/Follicle Stimulating Hormone (FSH) Ratio with Low Density Lipoprotein (LDL)/ High Density Lipoprotein (HDL) Ratio in Polycystic Ovarium Syndrome (PCOS) Patients Susi Oktaviani; Jusak Nugraha; Ashon Sa’adi
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 4 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i4.17106

Abstract

The aim of this study is to analyze the correlation between the ratio of LH and FSH levels with LDL andHDL cholesterol in PCOS patients. This study is an observational cross-sectional study. The population inthis study were women newly diagnosed with polycystic ovary syndrome who came to RSIA Ferina, RSIALombok 22, Elizabeth Clinic and RSIA Putri Surabaya and Clinical Pathology Laboratory of dr. SoetomoSurabaya. Examination of FSH, LH and LDL and HDL cholesterol levels on 27 specimens (stored frozenserum) using the ICT (Accurate Intan Madya®, VEDALAB) and enzymatic colorimetric (Dimension EXL®,Siemens) methods. Statistical analysis was performed using the Spearman and Pearson correlation test,with a significance level of p <0.05. The results showed that there was a moderate positive correlationbetween FSH and LDL cholesterol (r = 0.491, p = 0.009). Phenotype D of PCOS (non-hyperandrogenictype) had a moderate negative correlation between the LH / FSH ratio and the LDL/HDL cholesterol levelratio (r = -0.487, p <0.05). However, there was no relationship between the LH/FSH ratio and the ratioof LDL/HDL cholesterol levels in all SOPK patients in this study. This study showed that phenotype DPCOS patients (non-hyperandrogenic type) were less likely to have cardiovascular complications than otherphenotypic PCOS patients. Insulin resistance has a role in the risk of dyslipidemia in non-hyperandrogenicPCOS patients.
Qualitative Analysis of Cinnamomum burmannii Content using GCMS (Gas Chromatography Mass Spectometry) Method Hayati; Jusak Nugraha; Bambang Purwanto; Hari Basuki Notobroto; Yoes Prijatna Dachlan; Hari Setiono; Idha Kusumawati
Indian Journal of Forensic Medicine & Toxicology Vol. 16 No. 1 (2022): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v16i1.17556

Abstract

To identify the bioactive compounds in Cinnamomum burmannii and their biological activity. Cinnamonbark originating from Batu Malang, East Java, which was located 700-1300 meters above sea level, wasprocessed into dry extract by maceration method with 96% ethanol solvent. Furthermore, cinnamonethanol extract was analyzed using the GCMS method to look at the content of the bioactive componentfor further testing biological activity with the server Way2Drug PASS. GCMS results showed 40 activecompounds such as trans-cinnamaldehyde, trans-anethole, cinnamyl acetate, calacorene, cadina-1,4-diene, delta-cadinene. Furthermore, of the 40 compounds, the biological activity potential was testedfor 29 bioactive compounds based on PA (probable to be active) values predicted by the Way2DrugPASS server. Cinnamomum was tested against the potential as anti-fungi, anti-bacterial, anti-oxidant,anti-inflammatory, anti-diabetic, anti-neoplastic. Trans-cinnamaldehyde showed PA 0,583 as antiinflammatory, L-limonene PA0,818 as anti neoplastic, Tans-anethole PA 0,614 as anti neoplastic,Cinnamyl acetate PA 0,669 as anti inflammatory, calacorene PA 0,698 as anti inflammatory, DeltacadinenePA 0,651 as anti neoplastic, , Cathechin PA 0,828 as anti oxidant, alpha.-Cubebene PA 0,888 asanti inflammatory and PA 0,837 as anti neoplastic, melilotin PA 0,929 as anti neoplastic, CaryophyllenePA0,915 as anti neoplastic. Cinnamomum burmannii had biological potency based on potential activity(PA) 0,432 (+0,117) as antifungi, PA 0,335 (+0,090) as antibacterial, PA 0,304 (+0,199) as antioxidan,PA 0,561 (+0,190) as anti inflammatory, PA 0,373 (+0,170) as antidiabetic, PA 0,584 (+0,234) asantineoplastic.
Green Tea Suppresses Serum TNF-? and TGF-?1 Levels In Mice Model of Systemic Lupus Erythematosus Herin Mawarti; Jusak Nugraha; Djoko Agus Purwanto; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14746

Abstract

Objective: This study aims to investigate the effects of green tea administration on TNF-a, Hsp70, andTGF-b1 levels in the systemic lupus erythematosus (SLE). Material and methods: A total of 32 micewill be divided into four groups (each 8 mice), namely the control group, the SLE group, the SLE groupwho were given green tea extract at a dose of 500 mg/kg body weight, and the SLE group who were givengreen tea extract at a dose of 1000 mg/kg body weight. Analysis of TNF-a, Hsp70, and TGF-b1 levels wascarried out using the enzyme-linked immunosorbent assay technique. Results: TNF-a and TGF-b1 levelswere significantly increased in the SLE group compared to the control group (p < 0.05). This increase canbe significantly reduced through the provision of green tea, even reaching levels comparable to the controlgroup (p > 0.05). Conclusions: It was concluded that green tea containing EGCG can suppress TNF-a andTGF-b1 in the SLE model. Thus, green tea can be an alternative in immunology modulation in SLE.
Environmental Factors and Protective Effects of Epigallocatechin-3-Gallate to Systemic Lupus Eritematosus: A Review Study Herin Mawarti; Jusak Nugraha; Djoko Agus Purwanto; Joewono Soeroso
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 2 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i2.14750

Abstract

SLE (Systemic Lupus Erythematosus) is one of autoimmune disease in which the immune system attacksits own tissues, causing widespread inflammation and tissue damage in the affected organs. Etiology andpathogenesis are not clearly mention until now by the scientist, but environmental factor is contribute todevelopment of Lupus and Flare disease. The objective of this study to explain the relationship betweenenvironmental factors and pathogenesis development of SLE, and how the Epigallocatechin-3-Gallate(EGCG) can protect or prevent of organs damage and environmental effects.Method: The research design of this study was used literature review. The data sources collected fromElectronic database in Pubmed, Sage, Google Scholar and website of science including reports, journals andmostly published in the last 10 years.Results: The pathomechanism of SLE was influenced by environmental factors and this was caused anincreasing of oxidative stress, chronic inflammation, dysregulation of immune system and decreased theclean of immune complex and apoptosis of cells. The production of autoantibodies and immune complex arerelated with target tissue with is it was caused chronic inflammation and the end it caused irreversible damagein glomerulus of the kidneys, arteries, lungs and other tissues. EGCG plays a protecting role in environmentalfactors as a trigger because it works as an antioxidant, anti-inflammatory and immunomudulator. So EGCGcan as a potential agent to protect SLE.Conclusion: Environmental factors plays an important role as a triger of SLE and flare. Moreover, EGCG asa potential agent to protect the presence of oxidative stress, inflammation and immune dysregulation.
The Dynamics of Macrophage Function in Reparation of Natural Immune System in Tuberculosis Disease Christina Destri; Jusak Nugraha; Muhammad Amin; Djoko Agus Purwanto
Indian Journal of Forensic Medicine & Toxicology Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology
Publisher : Institute of Medico-legal Publications Pvt Ltd

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37506/ijfmt.v15i3.15720

Abstract

Macrophages related with pathogen was involved the ability of Mycobacterium tuberculosis (Mtb) to developing mechanisms to prevent macrophage attacks and the aim of this study was to find the relationship between specific role of macrophages in natural immune system in tuberculosis (TB) disease. The research design of this study used literature reviews from various journals and it was accessed in Google Scholar and various medical science websites who bublessed less than 10 years. Localization of mycobacteria in granulomas form and activation of macrophages ability to kill and eliminate pathogens also the movement of M1 to M2 polarization as an important part for the host to prevent widespread tissue damage. However, the loss of the immune system suppression by M1 and Th1 molecules will give benefit to fertility development of Mtb in the environment of immunity M2 / Th2. The results of this study showed that the dynamics of macrophages in tuberculosis disease is a balance of Mtb’s ability to escape from the immune system. The effectiveness of anti-TB treatment repair the immune system and eliminate Mtb
Aplikasi BGA UMAHA Sebagai Pendeteksi Gas Darah Disertai Interpretasi Hasil Berbasis Android Siti Nur Husnul Yusmiati; Anton Yuntarso; Jusak Nugraha
Jurnal SainHealth Vol 5, No 1 (2021): Maret 2021
Publisher : Faculty of Health Sciences Universitas Maarif Hasyim Latif

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51804/jsh.v5i1.897.8-17

Abstract

Derajat keasaman tubuh dapat mengalami peningkatan (alkalosis) dan penurunan (asidosis) akibat gangguan keseimbangan kation anion serta kegagalan transportasi dan produksi makromolekul sehingga permasalahan suatu fungsi sel dapat terjadi. Afinitas Ferro(Fe2+) yang terikat oleh molekul histidin dalam struktur haemoglobin terhadap oksigen akan terganggu jika pH darah arteri lebih kecil dari 7,35 atau lebih besar dari 7,45. Tingkat gangguan asam basa tubuh dapat diukur menggunakan alat gas darah. Pada saat pertama kali muncul alat gas darah hanya menyajikan hasil pH, pCO2, dan pO2dimana parameter yang lain seperti HCO3, BE, O2saturasi harus diplot terlebih dahulu menggunakan tabel Siggard Anderson. Perkembangan teknologi, mendukung terciptanya alat gas darah yang terintegrasi dengan logaritma bahasa program parameter hitungan, sehingga nilai HCO3,Base Excess Extra Cellular Fluid(BEecf), Base Excess Blood(BEB), O2 saturasi, dan parameter lain dapat termunculkan setelah didapatkan nilai pH, pCO2, dan pO2. Basis penelitian ini adalah pembuatan aplikasi mobile BGA UMAHA versi 0.1.0 yang dapat memberikan hasil interpretasi secara cepat, tepat, praktis, dan murah dengan tambahan inputan suhu tubuh dan kadar Hb pasien untuk mendapatkan nilai BEBase dan O2saturasi yang diperlukan, dengan demikian diharapkan permasalahan stabilitas alat, tingkat kelelahan klinisi dan efisiensi biaya dapat dilakukan. Kesimpulan pada penelitian ini adalah aplikasi mobile BGA UMAHA versi 0.1.0 dapat memunculkan parameter hitungan dengan inputan suhu dan kadar Hb dengan akurasi tinggi tanpa dipengaruhi nilai LOD alat serta dapat memunculkan interpretasi hasil.
Co-Authors Akhmad Setyo Rahman Anton Yuntarso Aryati Aryati Ashon Sa’adi Bambang Purwanto Bastian Betty Agustina Tambunan Ch. Destri Wiwis Wijayanti Christina Destri Citra Indah Setyaningrum Cyuzuzo Callixte Diyan Wahyu Kurniasari Djoko Agus Purwanto Dominicus Husada Eddy Mart Salim Elvan Dwi Widyadi Erna R Tobing Febtarini Rahmawati Ferdy R. Marpaung Ferdy Royland Marpaung Francisca Srioetami Tanoerahardjo Fransiska Sri Oetami Hari Basuki Notobroto Hari Setiono Herin Mawarti Hery Priyanto Idha Kusumawati Joewono Soeroso John Wiwin Kemas Ya’kub Rahadiyanto M. Aminuddin M. Irsan Saleh Maya E. Roring Muhammad Amin Muhammad Amminuddin Nova Kurniati Nur Hayati Nur Lailatul Fadhilah Risky V. Prasetyo S.Pd. M Kes I Ketut Sudiana . Sabrina Prihantika Shahzad Shoukat Siti Nur Husnul Yusmiati Soedarsono Soedarsono SP Edijanto Susi Oktaviani Theresia Indah Budhy Sulisetyawati Tuyishimire Irene Yoes Prijatna Dachlan Yulia Nadar Indrasari Zen Hafy