Pengaruh Waktu Thawing Terhadap Kualitas Spermatozoa Ayam Buras: The Effect of Thawing Time on the Quality of Native Chicken Spermatozoa

Siska Milanda (University of Muhammadiyah Sinjai)
Abdul Hakim Fattah (Universitas Muhammadiyah Sinjai)

Anoa: Journal of Animal Husbandry
Vol 1 No 1 (2022): Februari

The purpose of this study was to determine the effect of thawing time on the quality of native chicken spermatozoa. The research design used in this study was a completely randomized design (CRD) with 5 treatment levels and 5 replications. The treatments used were P1 = thawing time of 20 seconds, P2 = thawing time of 30 seconds, P3 = thawing time of 40 seconds, P4 = thawing time of 50 seconds, P5 = thawing time of 60 seconds. Semen that has been diluted to be packed in 5 ministraw 0.25 ml, then equilibrated for 2 hours. After equilibration, proceed with the freezing process (prefreezing). Then the straws were stored in a liquid N2 container (-196°C) for 24 hours. frozen semen was thawed again with warm water at 37°C according to the treatment. Parameters observed were sperm motility and recovery rate. The results of this study showed that thawing time had no significant effect (P>0.05) on sperm motility and recovery rate. It was concluded that the thawing time of 20 to 60 seconds could be used in freezing native chicken semen.