A design of a Polyethylene Terephthalate (PET) waste biodegradation system using Escherichia Coli (E-Coli) bacteria in a whole-cell biocatalyst system. E-Coli bacteria will produce LC-Cutinase enzyme on the cell surface so PET can be broken down into Ethylene glycol and Terephthalate acid. With the help of Reductase and Dehydrogenase enzymes, a chemical reaction occurs that converts Ethylene glycol into Malate. Through the chemical reaction process, it is guaranteed that Ethylene glycol does not explain the environment but can be used as an energy source for the growth of E-Coli bacteria. Thus E-Coli can grow faster, so the more bacteria, the more PET that can be broken down quickly.
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