Genetic Diversity of Duabanga moluccana Blume from Two Provenances in West Nusa Tenggara Revealed by Microsatellite markers

Siti Halimah Larekeng (Faculty of Forestry, Hasanuddin University)
Yusniar Yusniar (Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Universitas Hasanuddin, Makassar, Indonesia.)
Muh Restu (Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Universitas Hasanuddin, Makassar, Indonesia.)
Rismawati Rismawati (2nd Regional of Seed/Seedling Forest Tree, South Sulawesi, Indonesia)
Yuni Fitri Cahyaningsih (Indonesian Tropical Fruit Research Institute, Solok, West Sumatera, Indonesia.)
Mirza Arsiaty Arsyad (Faculty of Agrotechnology, Halu Oleo University, Kendari, Indonesia)
Arif Nirsatmanto (Centre for Forest Biotechnology and Tree Improvement (CFBTI), Yogyakarta, Indonesia)

International Journal of Agriculture System
VOLUME 8 ISSUE 1, JUNE 2020

Duabanga moluccana Blume, locally known as Rajumas, is a tree species that suitable for building materials, pulp, and plywood. The information about genetic diversity is required for the conserving of this species. Here, we elucidated the genetic diversity of D. moluccana from two provenances in West Nusa Tenggara, Indonesia. Four microsatellite markers successfully amplified 12 randomly selected samples and produced polymorphic DNA bands. Those primers were DMAG10, DMAG09, DMACAG01 and DMTCAC11. High genetic diversity was detected in the populations with 0.54 of mean expected heterozygosity (He). The genetic variation among individuals was 100%, whereas there were no genetic variations among populations and within individuals