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All Journal Jurnal Silvikultur Tropika (JST) Jurnal Natur Indonesia Tunas Geografi BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Jurnal Agroteknos JFMR (Journal of Fisheries and Marine Research) JURNAL GALUNG TROPIKA Indonesian Journal of Chemistry Majalah Farmasi dan Farmakologi (Trends in Pharmacy and Pharmaceutical Sciences) Martabe : Jurnal Pengabdian Kepada Masyarakat Forest and Society PERENNIAL Journal of Sylva Indonesiana CORD Buletin Palma Jurnal Pengabdian Dharma Wacana MALLOMO: Journal of Community Service Jurnal Pengabdian kepada Masyarakat Journal of Agriculture (JoA) International Journal of Agriculture System Perbal: Jurnal Pertanian Berkelanjutan Agrotech Journal ABDI UNISAP: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Forestry Research
SITI HALIMAH LAREKENG, SITI HALIMAH
Universitas Hasanuddin
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Library & Information Science Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Polimorfisme Penanda RAPD untuk Analisis Keragaman Genetik Pinusmerkusii di Hutan PendidikanUnhas Gusmiaty, Gusmiaty; Restu, Muh.; Asrianny, Asrianny; Larekeng, Siti Halimah
Jurnal Natur Indonesia Vol 16, No 2 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.822 KB) | DOI: 10.31258/jnat.16.2.47-53

Abstract

The aims of Genetic study of pinus identified stand in Unhas Experimental Forest is to analyses of genetic characteristics of stand, based on RAPD (Random Amplified Polymorphic DNA) marker. The study was conducted in Biotechnology and Tree Breeding Laboratory, Forestry Faculty, Hasanuddin University. The method are DNA isolation, Primers selection and RAPD analyses. DNA analyses of Pine with ten RAPD marker showed number of alel varietied and polymorphic. Coefficient of similarity in population have number of 0.15-0.73.Highest genetic distance is 0.9630 and lowest genetic distance is 0.2698. Number of genetic diversity of Pine in Experimental Forest Hasanuddin University is 0,489 and categorized highly.
KARAKTERISTIK STRUKTUR ANATOMI DINDING SEL JABON MERAH (Anthocephalus macrophyllus) PROVENANSI WAJO Nurul Muhlisah Mompewa; Andi Detti Yunianti; Siti Halimah Larekeng
PERENNIAL Vol. 15 No. 1 (2019)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v15i1.6496

Abstract

Apart from being a key to identifying wood, the characteristics of the anatomical structure of cell walls can be used as a reference in determining the quality of wood, especially for its use. However, the characteristics of wood in the same species are sometimes found to be different due to the growing place is unidentical. Therefore, this study aimed to identify the cell wall characteristics of Jabon Merah wood from Wajo provenance. The wood was collected from Tangkoro Village, Pitumpanua District, Wajo Regency, South Sulawesi. It was then prepared in slicing and maceration samples. The structures of cell walls observed in the preparations were a pit, perforation plate, and tylosis. The results showed the Jabon Merah had inter vessel pit and perforation plate types, namely polygonal alternating and scalariform, respectively. Also, it had a tylosis in the vessel.
Identifikasi dan Karakterisasi Mikoriza pada Tegakan Nyatoh (Palaquium sp.) Kurnia Kurnia; Gusmiaty Gusmiaty; Siti Halimah Larekeng
PERENNIAL Vol. 15 No. 1 (2019)
Publisher : Forestry Faculty of Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24259/perennial.v15i1.6850

Abstract

This research aimed to identify the mycorrhiza types that associated in roots and soil on  Palaquium sp. plant. This  research has implemented on December 2017 until February 2018. This research procedures made by two stages includes collecting soil and root samples on Palaquium sp plant on  Ko'mara Community Forest, Takalar District. Therefore, the second step is isolation, identification, and observing colonization of mycorrhiza spores in the Integrated Laboratory and Biotechnology and Tree Breeding Laboratory, Faculty of Forestry, Hasanuddin University, Makassar. The results showed types of spore that found were Glomus, Gigaspora and Acaulospora. Glomus spore has highest relative abundance in root samples whereas on soil samples were Glomus, Gigaspora, and Schleroderma with Glomus spores has highest relative abundance in. The colonization percentage on site was intermediate.
KERAGAMAN GENETIK IKAN KAKAP PUTIH (Lates calcarifer Bloch, 1790) TIPE LIAR DAN DOMESTIKASI MENGGUNAKAN METODE Random Amplified Polymorphic DNA (RAPD) Irmawati .; Basse Siang Parawansa; Asmi Citra Malina A.R Tassakka; Ma’rifa Baharuddin; Siti Halimah Larekeng
JFMR (Journal of Fisheries and Marine Research) Vol 5, No 1 (2021): JFMR VOL 5 NO.1
Publisher : JFMR (Journal of Fisheries and Marine Research)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jfmr.2021.005.01.15

Abstract

Kegiatan pembesaran ikan kakap putih di beberapa Kabupaten di Sulawesi Selatan yang menggunakan benih yang diproduksi dari balai perikanan di Sulawesi Selatan dan Ambon telah berkembang selama beberapa tahun terakhir. Akan tetapi, benih yang dihasilkan dari kegiatan pembenihan tersebut memiliki kualitas yang masih rendah yang diduga karena keragaman genetik yang rendah. Penelitian ini menggunakan metode Random Amplified Polymorphic DNA, RAPD untuk menganalisis variasi genetik ikan kakap putih tipe liar dari Bulungan Kalimantan Utara dan ikan hasil domestikasi dari panti pembenihan di Ambon yang dibesarkan di tambak rakyat di Desa Borongkalukua Kabupaten Maros Sulawesi Selatan. Hasil penelitian menunjukkan bahwa ikan kakap putih tipe liar memiliki nilai heterozigositas yang lebih tinggi dibandingkan dengan ikan kakap putih hasil domestikasi. Jarak genetik antar individu-individu di dalam populasi tipe liar lebih tinggi dibandingkan dengan jarak genetik individu-individu hasil domestikasi. Dendogram dan pohon filogenetik yang dibentuk berdasarkan evolutionary dissimilarity menunjukkan bahwa pada tingkat ketidakmiripan 10% ikan kakap putih tipe liar dari Bulungan Kalimantan Timur membentuk klaster yang terpisah dengan ikan kakap putih hasil domestikasi dari Ambon yang dipelihara di tambak Dusun Borongkalukua Kabupaten Maros. Keragaman genetik ikan hasil domestikasi lebih rendah dibandingkan ikan tipe liar dan menunjukkan pola evolusi yang pholyphyly Hasil yang diperoleh tersebut menunjukkan bahwa marka RAPD dapat digunakan untuk menganalisis perubahan keragaman genetik antara populasi ikan kakap putih tipe liar dan hasil domestikasi serta bermanfaat sebagai informasi dalam menyusun strategi pemanfaatan wild population sebagai sumber induk dan konservasi plasma nutfah ikan kakap putih.
OPTIMASI KOMBINASI NAA, BAP DAN GA3 PADA PLANLET KENTANG SECARA IN VITRO Siti Halimah Larekeng
JURNAL GALUNG TROPIKA Vol 1 No 1 (2012)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.93 KB) | DOI: 10.31850/jgt.v1i1.32

Abstract

Karakterisasi Cendawan Rhizosfer pada Tegakan Mahoni di Hutan Pendidikan Universitas Hasanuddin G Gusmiaty; Siti Halimah Larekeng
JURNAL GALUNG TROPIKA Vol 9 No 3 (2020)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v9i3.597

Abstract

Soil fungi, which produce hormones that occupy the rhizosphere ecosystem, have roles as agents to promote plant growth. This study aims to isolate rhizosphere fungi in mahogany in the Experiment Forest of Universitas Hasanuddin, and identify the fungal isolates obtained based on morphological characters. The research began with rhizosphere soil sampling, isolation, and identification based on morphological characters. The rhizosphere microbial isolation on mahogany stands in the Unhas Educational Forest obtained 18 fungal isolates with varied morphological characteristics, both in colony color and elevation. Microscopic observations resulted in 4 genera, namely Rhizopus, Gliocladium, Trichoderma, and Aspergillus. The results showed that there were superior fungi isolates that could produce growth hormones and could be applied to forest plant seeds. The formula with these select fungi can give the seeds optimal growth in forest plant seeds.
Aplikasi Variasi Konsentrasi Media MS dan Thidiazuron pada Induksi Kalus Pucuk Jati (Tectona grandis) Secara In Vitro Mirza Arsiaty Arsyad; Nur Reski Immalasari; Siti Halimah Larekeng
JURNAL GALUNG TROPIKA Vol 9 No 2 (2020)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v9i2.627

Abstract

Informasi Genetik Bitti (Vitex cofassus) di Areal Sumber Daya Genetik (ASDG) BPTH Wilayah II Sulawesi Siti Halimah Larekeng; Zulfadilah Syam; M Musriati; Rathna Paelongan; Astuti Arif; Muhammad Restu; I Iswanto
JURNAL GALUNG TROPIKA Vol 10 No 3 (2021)
Publisher : Fapetrik-UMPAR

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31850/jgt.v10i3.801

Abstract

Bitti (Vitex cofassus) is one of the most important tree species in Sulawesi, which is used as material for phinisi boats. The aim study was to study the genetic diversity of Bitti in the genetic resources area in Bone Regency. This study used provenance Bulukumba, Muna, Kolaka, and North Luwu proof which was analyzed based on microsatellite markers. The resulting study showed evidence that provenance Muna has a Ho value 0f 0,65 or a relatively high genetic diversity value in comparison to provenance Bulukumba, Kolaka, and North Luwu.
POLLEN DISPERSAL PATTERNS EBONI LASITAE PROVENANCE BASED ON SIMPLE SEQUENCE REPEATS (SSR) Muhammad Restu; Gusmiaty .; Siti Halimah Larekeng
Jurnal Silvikultur Tropika Vol. 7 No. 3 (2016): Jurnal Silvikultur Tropika (Suplemen Desember)
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.7.3.S31-S35

Abstract

Parentage analysis has been used to evaluate pollen dispersal in ebony (Diosphyros celebica Bakh.). The objectives of this research were to evaluate (i) the dispersal of pollen, and (ii) the distance of pollen travel in ebony Lasitae provenances. The finding of this activities should be beneficial to support breeding of this ebony tree. There were 14 progeny arrays were harvested from 3 female parents. There were 32 ebony trees surrounding the female parents were analyses as the potential male parents for the progenies. Ebony tress were mapped according to their GPS position. All samples were genotyped using four SSR marker loci. Parentage analysis was done using CERVUS version 2.0 software. Results of the analysis indicated the evaluated markers were effective for assigning candidate male parents to all evaluated seedlings. There is no specific direction of donated pollen movement from assigned donor parents to the female ones. The donated pollens could come from assigned male parents in any directions relative to the female parent positions. Pollen dispersal pattern of ebony female parent occured in outcrossing pollination among its different donated trees. Based on the progenies analysis, ebony female parent is a dominantly outcrossing pollination species. Distances of pollen travel reach to 18 until 269 meter.Key words: Ebony, ssr marker, pollen dispersal.
HIGH OUTCROSSING RATE AND POLLEN DISPERSAL DISTANCE of Diospyros celebica Bakh. (EBENACEAE), AN ENDEMIC TREE SPECIES IN SULAWESI ISLAND, INDONESIA Muhammad Restu; Gusmiaty G.; Siti Halimah Larekeng
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 3 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2017.24.3.562

Abstract

Diosphyros celebica Bakh., also known as Sulawesi ebony, is an endemic to central and northern Sulawesi. Information about pollen dispersal patterns of D. celebica have not been previously investigated. This study was aimed to determine pollination type, percentage of selfing and outcrossing, as well as distance of pollen dispersal of D. celebica. This study was conducted at experimental forest of Universitas Hasanuddin, Maros District, South Sulawesi Province, Indonesia. One hundred and sixty six individuals of D. celebica consisted of adult trees and seedlings were analyzed in this study. Ninety four adult trees were selected to become parent trees. The trees were mapped with GPS coordinates . All samples were genotyped using four SSR markers loci. Parental analysis and determination of pollen dispersal patterns were carried out using Cervus 3.0.3. Results indicated that the evaluated markers were effective for assigning candidate of male parents to all evaluated seedlings. Donated pollens could come from male parents in any directions relative to female parent positions. Pollen dispersal pattern showed outcrossing pollination among different male parents (pollen donated trees). The results indicated that seeds were produced predominatly by outcrossing. Pollen dispersal reached up to 166 m. Pollen related processes were linked through female parents, pollinators availability and ecological environment. Simultaneous use of progeny genotyping, spatially explicit analysis of environmental variables and outcomes of plant–animal interactions, were the key elements for an expanded approach to gene flow analysis considering dispersal via pollen and seeds. Research on pollen dispersal of D. celebica should be carried out in other forest types, such as mixed forest and highland forest.
Co-Authors A Mujetahid A.R Tassakka, Asmi Citra Malina Abdul Karim Abdur Rahman Arif Adnan Adnan Agus Purwito Agus Rachmat Ahyar Ahmad Ahyar Ardat, Muh Andi Detti Yunianti Andi Detti Yunianti Andi Erwin Eka Putra Andi Prastiyo Andi Siady Hamzah Andri Ardiansyah Andri Ardiansyah Anila, Chaeria Anita Anita Arfah, Rugaiyah Andi Arif Nirsatmanto Aris Prio Ambodo Aris Prio Ambodo Arsyad, Mirza Arsiaty Aslan, Aura Aulia Asmi Citra Malina Asrianny Asrianny, Asrianny Asriyanni Asriyanni Astari Rusdin, Rista Astuti Arif Baharuddin, Ma’rifa Basse Siang Parawansa Budiaman Budiaman Budiman, Yogi Anang Budirman Bachtiar Budirman Bahtiar Dirpan, Andi Dirvamena Boer DIRVAMENA BOER, DIRVAMENA Dr. Herlina Rante, M.Si., Apt DWI SURYANTO Ekajayanti Kining ELIHAMI, ELIHAMI Fa'iq, Muhammad Feranita Haring G Gusmiaty Gusmiati Gusmiaty . Gusmiaty G. Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty Gusmiaty, Gusmiaty Hairil Adzulyatno Hadini Hajrawati, Hajrawati Harningsih Karim Hasnah Natsir Hisein, Waode Siti Anima I Irwan I Iswanto I. Iswanto Irfandi, Rizal Irmawati . Irmawati Irmawati ISMAIL MASKROMO Ismail Maskromo Iswanto Iswanto Joeharnani Tresnati Khairana Kadriah, Ince Ayu Kurnia Kurnia M Musriati M. Yunus Sudirman Marlina Mustafa, Marlina Marwan Rajab Matjik, Nurhayati Anshori Ma’rifa Baharuddin Michely Jauwdy Stevic Mirza Arsiaty Arsyad Mirza Arsiaty Arsyad Mirza Arsiaty Arsyad Mochammad Imron Awalludin Muh Alif KS Muh Restu Muh. Farid Muh. Restu Muh. Restu Muh. Restu Muh. Restu Muhammad Bima Akzad Muhammad Fuad Anshori Muhammad Restu Muhammad Restu Munajat Nursaputra, Munajat Mustari, Andi Subhan Nasir, Anugrahandini Nasri Nasri Nawir Nawir, Nawir Negara, Satria Putra Jaya Nur Reski Immalasari NurAfni NurAfni Nurfadilah Latif NURHAYATI ANSHORI MATTJIK Nurul Muhlisah Mompewa Parawansa, Basse Siang Paulina Taba Prof. Dr. Gemini Alam, M.Si., Apt Rahayu, Rifqah Rahmat, Syaeful Ramadani, Ananda Rathna Paelongan Rayu, Surya Muh. Furqan Rehan Rehan Restu, Muhammad Rifdah Anggrini Zabir Riksfardini Annisa ERMAWAR Rismawati Rismawati Rismawati Rismawati S. Sudarsono Saidil, Saidil Sarlan, Sarlan Siti Aslamyah Sitti Nuraeni Suaib, Suaib SUDARSONO SUDARSONO Suharman Suharman, Suharman Sukriati A. Lamanda Supratman Supratman Suriati Eka Putri Syahriani Syahriani, Syahriani Syamsuddin Millang Yohan Lawang Yohan Lawang Yuni Fitri Cahyaningsih Yuni Fitri Cahyaningsih Yuni Fitri Cahyaningsih Yusniar Yusniar Zulfadilah Syam