Integrated Lab Journal
Vol. 10 No. 02 (2022): Integrated Lab Journal

Identifikasi Bakteri Kontaminan Pada Kultur Jaringan Bambu Jenis Fargesia scabrida

Erna Wulandari (UIN Sunan Kalijaga Yogyakarta)



Article Info

Publish Date
31 Oct 2022

Abstract

Contaminant microbes are a common problem in plant tissue culture. Bacteria is a type of microbial that becomes a contaminant in plant tissue culture. This study aims to isolate and determine the diversity of bacterial contaminants of Fargesia scabrida bamboo tissue culture. In this study, bacterial isolation, phenotypic characterization included morphology, physiological tests, biochemical tests, identification, and phenetic numerical analysis were carried out. The results of this study indicated that 7 bacterial isolates were obtained. Based on profile matching using Bergey's Manual of Determinative Bacteriology, 1 isolate belongs to the genus Bacillus, 1 isolate belongs to Listeria, 2 isolates Erwinia, 2 isolates Lactobacillus, and 1 isolate belongs to the genus Klebsiella. The dendogram generated from the Simple Matching coefficient (SSM) parameter using the average linkage algorithm shows that at a similarity value of 70%, it is divided into 5 clusters. While the dendrogram generated from the SJ parameters using the average linkage/UPGMA at a similarity value of 70% 7 OTUs are divided into 7 clusters.

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Journal Info

Abbrev

integratedlab

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Engineering Industrial & Manufacturing Engineering

Description

Integrated Lab Journal published by Sunan Kalijaga State Islamic University of Yogyakarta as a media for scientific paper about educational laboratories. The focus and scope of journal is about : 1. Educational Laboratories Tools, Equipment, or Machine : Maintenace and safety Procedures and ...