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All Journal Biology, Medicine, & Natural Product Chemistry Integrated Lab Journal
Erna Wulandari
UIN Sunan Kalijaga Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Engineering Industrial & Manufacturing Engineering Medicine & Pharmacology Public Health

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Identifikasi Bakteri Kontaminan Pada Kultur Jaringan Bambu Jenis Fargesia scabrida Erna Wulandari
Integrated Lab Journal Vol. 10 No. 02 (2022): Integrated Lab Journal
Publisher : UIN Sunan Kalijaga Yogyakarta

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Abstract

Contaminant microbes are a common problem in plant tissue culture. Bacteria is a type of microbial that becomes a contaminant in plant tissue culture. This study aims to isolate and determine the diversity of bacterial contaminants of Fargesia scabrida bamboo tissue culture. In this study, bacterial isolation, phenotypic characterization included morphology, physiological tests, biochemical tests, identification, and phenetic numerical analysis were carried out. The results of this study indicated that 7 bacterial isolates were obtained. Based on profile matching using Bergey's Manual of Determinative Bacteriology, 1 isolate belongs to the genus Bacillus, 1 isolate belongs to Listeria, 2 isolates Erwinia, 2 isolates Lactobacillus, and 1 isolate belongs to the genus Klebsiella. The dendogram generated from the Simple Matching coefficient (SSM) parameter using the average linkage algorithm shows that at a similarity value of 70%, it is divided into 5 clusters. While the dendrogram generated from the SJ parameters using the average linkage/UPGMA at a similarity value of 70% 7 OTUs are divided into 7 clusters.
Molecular Phylogenetic Analysis of 16S rRNA Gene of Facultative Hetero-Fermentative Lactic Acid Bacteria Habib Ramadhan; Erna Wulandari
Biology, Medicine, & Natural Product Chemistry Vol 15, No 1 (2026)
Publisher : Sunan Kalijaga State Islamic University & Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14421/biomedich.2026.151.1641-1647

Abstract

Facultative heterofermentative lactic acid bacteria are bacteria that ferment carbohydrates to produce lactic acid and by-products such as acetic acid, ethanol, and CO2. This group of bacteria is widely used in the food and health industries. However, research on BAL species, especially the facultative heterofermentative group, is still relatively limited, so further studies are needed with molecular phylogenetic analysis aimed at providing an overview of the kinship of BAL species in the facultative heterofermentative group using the 16S rRNA gene molecular marker for more accurate identification results. The research design was conducted as a molecular-based descriptive analysis using secondary data in the form of 16S rRNA gene nucleotide sequences taken from the National Center for Biotechnology Information (NCBI) GenBank. Data analysis was performed using bioinformatics tools, including sequence alignment using ClustalX, phylogenetic tree reconstruction using MEGA and PHYLIP, and similarity matrix analysis using PHYDIT. The results of the analysis showed that Lactobacillus casei and Lactobacillus rhamnosus, as well as Lactobacillus pentosus and Lactobacillus plantarum, were closely related with a bootstrap value of 100% and a similarity matrix of 98-99%. Meanwhile, Lactobacillus alimentarius showed a relatively close relationship with Lactobacillus pentosus and Lactobacillus plantarum with bootstrap values and similarity matrices of around 92%. Thus, molecular phylogenetic analysis is effective in analyzing, identifying, and proving the relationship between BALs of the facultative heterofermentative group.
Co-Authors Habib Ramadhan