Current Research on Biosciences and Biotechnology
Vol. 4 No. 1 (2022)

Total phenolic, flavonoid, and antioxidant capacity of bajakah (Spatholobus littoralis Hassk)

Muhammad Ikhlas Arsul (Unknown)
Nurfajri Syamsi (Alauddin Islamic State University)
Nadila Putri (Alauddin Islamic State University)
Nur Afiah Alfrianti Nur (Alauddin Islamic State University)
Mukhriani (Alauddin Islamic State University)
Nursalam Hamzah (Alauddin Islamic State University)



Article Info

Publish Date
31 Aug 2022

Abstract

The bajakah (Spatholobus littoralis Hassk.) is one of the typical plants from Kalimantan Island, which has potential as a drug, one of which using as an antioxidant is due to the secondary metabolites contained therein. The aims of the research are to estimate the total phenolic content, total flavonoid content, and antioxidant capacity of the bajakah by different methods. Extraction was carried out by reflux method using a solvent gradient of n-hexane, ethyl acetate, and ethanol 96%. Phenols and flavonoid content were calculated using spectroscopy. Antioxidant capacity was measured against ascorbic acid and Trolox using the CUPRAC, DPPH, and FRAP methods and reported as mg Trolox equivalent (TEAC) and ascorbic acid equivalent (AEAC) per gram extract. The ethanol extract showed the highest antioxidant capacity for the CUPRAC and FRAP methods, the n-hexane extract for the DPPH method on both varieties of bajakah tampala. Each method provides significantly different antioxidant capacity values. The CUPRAC and FRAP methods found the highest antioxidant capacity in the white variety of bajakah ethanol extract. Meanwhile, in the DPPH method, saw the highest antioxidant capacity in the red variety of n-hexane bajakah extract. DPPH and CUPRAC give a positive correlation with TPC, where CUPRAC assay produced higher values than FRAP and DPPH assays.

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Journal Info

Abbrev

crbb

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Materials Science & Nanotechnology Medicine & Pharmacology

Description

The Current Research on Biosciences and Biotechnology (CRBB) encourages researchers to publish works related to the use of the biosciences and biotechnology in understanding the world around us. From Health and Medicine to Advanced Materials, these state-of-the-art research offer novel insights in a ...