This study was conducted to evaluate the fertility of swamp buffalo synchronized by prostaglandin administration prior to both natural mating and artificial inse-mination (AI). Thirty six swamp buffalo cows were intramuscularly injected by 35 mg prostaglandin (Lutalyse®, Pfizer, Puurs, Belgium) twice in eleven consecutive days. The buffaloes that exhibit estrous signs allotted into 2 treatments i.e. T1 (natural mating) and T2 (AI). Next to second prostaglandin injection, T1 cows were caged together with bull at 72 h for 2 d, and T2 cows were artificially inseminated at 72 h and 96 h. All cows were observed for next estrous emergence determining non return status. Pregnancy was examined 60 days after insemination by recto-vaginal palpation. The variables measured were non return rate (NRR) and concep-tion rate (CR), analyzed statistically using chi-square test. The results showed that NRR and CR of T1 and T2 had no significant difference. The NRR of T1 and T2 were 88.9% and 83.3% respectively, CR were 72.2% (T1) and 55.6% (T2). In conclusion, natural mating and AI give equal fertility to estrous synchronized swamp buffalo.
                        
                        
                        
                        
                            
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