Avocado leaves are a source of flavonoid active compounds, quercetin, and polyphenols that function as antioxidants. Flavonoids are secondary metabolites consisting of a group of polyphenolic compounds that have a pyron structure. Antioxidants are needed as an antidote to free radicals, antioxidants are needed by the body to neutralize free radicals and also to prevent damage caused by free radicals. This research was conducted using a pre-experimental method with a one-shot case study design using avocado leaf sampels growing in Blitar village and Tulungagung village. Determination of antioxidant activity testing was carried out using the DPPH method which was measured using a Uv-Vis spectrophotometer. The results of the extracts and the results of antioxidant activity obtained by the ethanol extract of avocado leaves in Blitar village with IC₅₀ value of 114.0851 ppm and Tulungagung village with IC₅₀ value of 99.2852 ppm. Blitar avocado leaf ethanol extract has an IC value of 114.0851 ppm and Tulungagung avocado leaf ethanol extract has an IC value of 99.2852 ppm. And it can be concluded that the ethanol extract of avocado leaves (Tulungagung) in the highlands has a stronger antioxidant activity than the ethanolic extract of avocado leaves (Blitar) in the lowlands.
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