Isolation and Identification of β-sitosterol, 7-hydroxystigmast-22-en-3,6-dione and 3β, 24(S)-dihydroxycholesta-5, 25-diene-7-one from stem bark of Nauclea pobeguinii

Adewusi John Adepoju (Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State)
Sunday Olusegun Oladoye (Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State)
Ezekiel Temidayo Ayodele (Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State)
Adeola Victoria Falade (Organic Chemistry Unit, Department of Pure and Applied Chemistry, Ladoke Akintola University of Technology, Ogbomoso, Oyo State)
Gin Keat Lim (School of Chemical Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang)
Chuan Wei OO (School of Chemical Sciences, Universiti Sains Malaysia, 11800 USM, Pulau Pinang)

Majalah Obat Tradisional
Vol 27, No 3 (2022)

The stem bark of the Nauclea pobeguinii was collected, air-dried, and pulverized and was extracted with solvent of varying polarity (n-hexane, ethyl acetate, and ethanol) to obtain the crude extracts. Silica gel column and thin layer chromatographic separation afforded three compounds whose structures were elucidated as β-sitosterol (1), 7-hydroxystigmast-22-en-3.6-dione (2), and 3β, 24(S)-dihydroxycholesta-5, 25-dien-7-one (3) by analysis of their chemical and spectral characteristic from 1D and 2DNMR, FTIR and by comparing of data with those reported in the literature.