TESTING THE EFFECTIVENESS OF LIME (Citrus aurantifolia.S) JUICE AS A NATURAL HAND SANITIZER AGAINST THE GROWTH INHIBITORY OF THE BACTERIA Acinetobacter baumannii

Soeyati Poejiani (Brawijaya University)

Indonesian Journal of Laboratory
Vol 5 No 3 Tahun 2022 (in Press)

Lime juice contains several main active ingredients that can act as antibacterial including flavonoids, citric acid, and limonene. Acinetobacter baumannii is a Gram-negative rod that is aerobic, immobile or non-motile. This study aims to determine the effectiveness of lime juice as a natural hand sanitizer against the growth inhibition of Acinetobacter baumannii. The research method used is the disc diffusion method. The antibacterial activity of lime juice was determined by the minimum inhibitory concentration (MIC). The concentration variations used were 10%, 20%, 30%, 40%, 50%, 0% (negative control), 10µg Meropenem dish (positive control). Based on the research obtained MIC of lime juice at a concentration of 10%. Inhibition zone test data were analyzed using One Way Annova. The results showed that the MIC of lime juice at a concentration of 10% showed an inhibition zone of 7.74 mm wider than the antibiotic Meropenem 10µg with an inhibition zone of 6 mm.