Proceeding ISETH (International Summit on Science, Technology, and Humanity)
2016: Proceeding ISETH (International Conference on Science, Technology, and Humanity)

The Potency of Kitolod (Isotoma longiflora (L)Presl.) Herb Extract as a Cure for Cervical Cancer: an in Vitro Study of Hela Cells

Atikah Hapsari (Universitas Muhammadiyah Surakarta)
Dhinar Asti (Universitas Muhammadiyah Surakarta)
S Selviana (Universitas Muhammadiyah Surakarta)
Ria Hidayati (Universitas Muhammadiyah Surakarta)
Novea Kumalla (Universitas Muhammadiyah Surakarta)
Andi Suhendi (Universitas Muhammadiyah Surakarta)



Article Info

Publish Date
01 Aug 2016

Abstract

Cervical cancer is the leading cause of death in women in the world. Cases of cervical cancer are the majority causes of death occurring in developing countries. The existing medicines which have a lot of functions as anticancer, however, still can damage the normal cells in our body. Cancer treatment using chemotherapy also has some side effects. The condition encourages researchers to explore anticancer cure made of natural ingredient. This research was conducted to find the potency of ethanol extract of herb kitolod (Isotoma longiflora (L) Presl.) as a cervical cancer cure to heLa cell. The method being used for the cytotoxic test on HeLa cells was MTT assay. Absorbant performed by ELISA reader at a wavelength of 595nm. Phytochemical screening which had been conducted showed a significant result that kitolod plants contain secondary metabolites compounds such as flavonoids, steroids, saponins, tannins and alkaloids. The results of cytotoxic test of the kitolod herb ethanol extract on HeLa cells by using MTT assay method acquired a value of IC50 of 227 μg/mL. This study showed that the ethanol extract of the herb kitolod had the potency to inhibit cervical cancer cells (HeLa cells) in a moderate ability.

Copyrights © 2016






Journal Info

Abbrev

iseth

Publisher

Subject

Computer Science & IT Engineering Medicine & Pharmacology Social Sciences Other

Description

The International Summit on Science, Technology and Humanity (ISETH) is organised by Universitas Muhammadiyah Surakarta. This summit aims to provide a platform for researchers and academics to share their research findings with others and meet lecturers and researchers from other institutions and to ...