Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Hapsari, Atikah
Unknown Affiliation
Computer Science & IT Engineering Medicine & Pharmacology Social Sciences Other

Published : 2 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 2 Documents
Search

 1 
Cytotoxicity of Ethanol Extract, Polar, Semipolar, and Nonpolar Herb Citolod (Isotoma longiflora (L.) C. Presl.) Cells on MCF-7 Cells Haryoto, H; Hapsari, Atikah
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2019: Proceeding ISETH (International Summit on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.1432

Abstract

Breast cancer is a major cancer suffered and the most cause of death by women in the world. The existing drugs of anticancer are not selective, disturbing the growth of normal cells. It encourages researchers to explore a chemopreventive agent from the nature materials. This study was conducted to determine the cytotoxic activity of ethanol extract, polar fractions, semipolar, and nonpolar herb chitolod (Isotoma longiflora (L) Presl.) on MCF-7 cells. First, herb chitolod was dried, refined, and sieved using mesh number 40 and then macerated using 96% ethanol for 72 hours. Phytochemical screening was conducted with TLC using GF254 silica as stationary phase, n-hexane: ethyl acetate (7: 3) as mobile phase, then the result was visualized on UV 254 nm, 366 nm, and visible light. Anisaldehid, dragendroff, sitoborat, and FeCl3 reagents spray were used as visualizer of TLC spot results. Fractination using KCV method, then cytotoxic test using MTT assay. A result obtained, the ethanol extract of herb chitolod positively contains flavonoids, phenolics, alkaloids, terpenoids, saponins, steroids and tannins. IC50 value of the ethanol extract, polar fractions, semipolar, and nonpolar are 521,05; 213,29; 499,94; and 239,43 µg/mL. This study showed that the ethanol extract, polar fraction, semipolar, and nonpolar of herb chitolod had moderate cytotoxic activity on MCF-7 cells.
The Potency of Kitolod (Isotoma longiflora (L)Presl.) Herb Extract as a Cure for Cervical Cancer: an in Vitro Study of Hela Cells Hapsari, Atikah; Asti, Dhinar; Selviana, S; Hidayati, Ria; Kumalla, Novea; Suhendi, Andi
Proceeding ISETH (International Summit on Science, Technology, and Humanity) 2016: Proceeding ISETH (International Conference on Science, Technology, and Humanity)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/iseth.2359

Abstract

Cervical cancer is the leading cause of death in women in the world. Cases of cervical cancer are the majority causes of death occurring in developing countries. The existing medicines which have a lot of functions as anticancer, however, still can damage the normal cells in our body. Cancer treatment using chemotherapy also has some side effects. The condition encourages researchers to explore anticancer cure made of natural ingredient. This research was conducted to find the potency of ethanol extract of herb kitolod (Isotoma longiflora (L) Presl.) as a cervical cancer cure to heLa cell. The method being used for the cytotoxic test on HeLa cells was MTT assay. Absorbant performed by ELISA reader at a wavelength of 595nm. Phytochemical screening which had been conducted showed a significant result that kitolod plants contain secondary metabolites compounds such as flavonoids, steroids, saponins, tannins and alkaloids. The results of cytotoxic test of the kitolod herb ethanol extract on HeLa cells by using MTT assay method acquired a value of IC50 of 227 μg/mL. This study showed that the ethanol extract of the herb kitolod had the potency to inhibit cervical cancer cells (HeLa cells) in a moderate ability.
Co-Authors Andi Suhendi Asti, Dhinar Haryoto, H Hidayati, Ria Kumalla, Novea Selviana, S