Journal of Tropical Biodiversity and Biotechnology
Vol 8, No 1 (2023): April

Attacus atlas (L.) sericin extract as an effective UV Protectant of Bacillus thuringiensis serotype kurstaki for controlling Spodoptera litura (Fab.)

Nindita Sabila Ningtyas (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Rahmatullah Rahmatullah (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Aryo Seto Pandu Wiranto (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Nadya Sofia Siti Sa’adah (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Hipny Alwandri (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Asma’ Asma’ (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Tiara Purti Salsabila (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Hanindyo Adi (Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)
Sukirno Sukirno (Entomology Laboratory, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia)



Article Info

Publish Date
03 Jan 2023

Abstract

Bacillus thuringiensis serotype kurstaki is an entomopathogenic bacteria commonly used to control the cutworm Spodoptera litura (Fab.). However, B. thuringiensis has disadvantage of being easily degraded due to exposed sunlight. The objective of this research was to determine the effectiveness of adding A. atlas (L.) cocoon extract as UV protectant B. thuringiensis to the mortality of S. litura. This research formulated 2.5% of the original substance of A. atlas cocoon extract and B. thuringiensis serotype kurstaki strain HD-7 applied from commercial product DiPel-WP®. The formulation was exposed to sunlight for 0, 1, 2, and 3 weeks. The suspension treated for 20 individuals of first instar larvae S. litura shifted into the artificial diet using 3-5 replicates. The scanning electron microscope (SEM) method began from a sample that was vacuumed, sample coated, and observed on SEM with the electron in a certain level probe. This research showed that the mortality of S. litura decreased with the growth of S. litura. The mortality of S. litura achieved 20%-100% mortality after treatments. The A. atlas cocoon extract was effective as UV protectant B. thuringiensis for three weeks of exposure to sunlight. The SEM analysis represented that formulation of B. thuringiensis and A. atlas cocoon extract sunlight exposure for one week has harsher surface than exposed during three weeks.  

Copyrights © 2023






Journal Info

Abbrev

jtbb

Publisher

Subject

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

Description

Journal of Tropical Biodiversity and Biotechnology (JTBB) is an authoritative source of information concerned with the advancement of tropical biology studies in the Southeast Asia Region. It publishes original scientific work related to a diverse range of fields in tropical biodiversity, functional ...